GEO DataSets

(Submitter supplied) Kc167 Cells were plated at 0.5x106/ml and grown at 23.50C. They were treated with 20-HE (Sigma) at a final concentration of 5x10-7M. The reference sample was total RNA isolated at 0 hr. after treatment. The experimental samples were total RNA isolated from cells treated for 1, 3, 6, 12, 24, and 48 hours.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6912

32 Samples

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(Submitter supplied) We used the DamID method to systematically identify the binding sites of Ecdysone Receptor and its heterodimeric partner USP across the whole genome in Drosophila Kc cells. We find that the EcR sites are a subset of the USP sites and that only a proportion are ecdysone regulated from an accompanying ecdysone profiling study. The role of EcR/USP in the ecdysone network appears to be coordinated by the recruitment of many transcription factors as well as signaling molecules. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL1447

12 Samples

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(Submitter supplied) We used microarray analysis of RNA obtained from w1118 and w1118;sox14L1/sox14L1 animals staged at pupariation to identify genes regulated by Drosophila Sox14 at the onset of metamorphosis.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

6 Samples

Download data: CEL

(Submitter supplied) This study identifies those genes that are dependent on EcR for their proper regulation at the onset of metamorphosis in Drosophila melanogaster. Keywords: Nuclear receptor gene regulation

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2675

Platform:

GPL72

18 Samples

Download data: CEL

(Submitter supplied) To identify 20E-regulated genes, wandering third instar larvae were dissected and their organs were cultured in the presence of either no hormone, 20E alone, cycloheximide alone, or 20E plus cycloheximide for six hours. Keywords: Hormone response

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2674

Platform:

GPL72

12 Samples

Download data: CEL

(Submitter supplied) This study identifies genes that alter their expression in synchrony with the late third instar and prepupal pulses of 20E. Keywords: time course

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2673

Platform:

GPL72

27 Samples

Download data: CEL

Analysis of ecdysone receptor (EcR) depleted animals 4 hours before to 4 hours after the start of pupariation. EcR is part of receptor complex for 20-hydroxyecdysone (20E), a hormone that triggers developmental transitions in insects. Results provide insight into the mechanisms regulated by 20E.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 development stage, 2 protocol, 3 time sets

Platform:

GPL72

Series:

GSE3069

18 Samples

Download data: CEL

Analysis of cultured larval organs treated with 20-hydroxyecdysone (20E) alone or in combination with cycloheximide to distinguish primary responses to 20E signal. Organs dissected from third instar larvae. Results provide insight into the molecular events triggered by 20E during insect development.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL72

Series:

GSE3060

12 Samples

Download data: CEL

Analysis of animals before and after pupariation. A pulse of steroid hormone 20-hydroxyecdysone (20E) at late 3rd instar triggers puparium formation; a second 20E pulse after pupariation marks the prepupal-to-pupal transition. Results provide insight into the molecular mechanisms of 20E signaling.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 3 development stage, 9 time sets

Platform:

GPL72

Series:

GSE3057

27 Samples

Download data: CEL

(Submitter supplied) Steroid hormones regulate tissue development and physiology by modulating the transcription of a broad spectrum of genes. In insects, the principal steroid hormones, ecdysteroids, trigger the expression of thousands of genes through a cascade of transcription factors (TFs) to coordinate developmental transitions such as larval molting and metamorphosis. However, whether ecdysteroid signaling can bypass transcriptional hierarchies to exert its function in individual developmental processes is unclear. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30203

6 Samples

Download data: TAB, TXT

(Submitter supplied) The subject of the current study is the finding of possible molecular partners of drosophila EcR receptor. The whole-genome experiments revealed that the sites of EcR receptor are partially overlapped with ERR binding sites. As ERR receptor specifically binds regulatory regions of glycolytic genes and genes of glycogen metabolism, the presence of EcR on ERR targets signifies involvement of the ecdysone signaling in regulation of carbohydrate metabolism.

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21306

4 Samples

Download data: BIGWIG

(Submitter supplied) Ecdysone signaling has long been studied as a paradigm for how hormones can synchronize development events in tissues throughout the animal. At the genetic level, ecdysone acts through its receptor, EcR, which has been classically thought to act at a relatively small number of canonical, ecdysone-responsive genes which then go on to activate the effectors of cellular processes. However, in spite of the decades of work studying EcR, the understanding of how EcR promotes changes in gene expression genome-wide in vivo remains incomplete. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL16479 GPL17275

12 Samples

Download data: BED, BW, CSV

(Submitter supplied) In Drosophila, male-specific FRU (FRUM) is required to establish the potential for courtship behaviors, but the downstream effectors of FRUM during development are largely unknown. A microarray-based approach identified genes that are differentially expressed as a consequence of FRUM in pupae, in both whole body and CNS tissues. Genes were also identified that are sex-differentially expressed in CNS tissues. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6799

30 Samples

Download data: GPR

(Submitter supplied) The subject of the current study is the finding of possible molecular partners of Drosophila EcR receptor. Two labelling enzymes (BioID2 and APEX2) were fused to EcR or Usp to biotin label the surrounding proteins. All fused proteins were expressed using the Act5C promoter in Drosophila S2 cells. To ensure functionality of the generated proteins, we verified their ability to bind EcR and Usp sites in the Drosophila genome with the ChIP-Seq. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25244

17 Samples

Download data: BIGWIG

(Submitter supplied) Transcriptional profiling of 16-20 hrs Drosophila embryos comparing control (w1118) and DHR3 mutants (DHR3G60S/Df(2R)12)

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL7300

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL23702 GPL17275 GPL25244

48 Samples

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(Submitter supplied) In Drosophila, the ecdysone steroid hormone is essential for coordinating developmental timing across physically separated tissues. Ecdysone directly impacts genome function through its receptor, a heterodimer of the EcR and Usp proteins. Ligand binding to EcR triggers a transcriptional cascade, including activation of a set of primary response transcription factors. The hierarchical organization of this pathway has left the direct role of EcR in mediating ecdysone responses obscured. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23702

20 Samples

Download data: CSV, XLSX

(Submitter supplied) In Drosophila, the ecdysone steroid hormone is essential for coordinating developmental timing across physically separated tissues. Ecdysone directly impacts genome function through its receptor, a heterodimer of the EcR and Usp proteins. Ligand binding to EcR triggers a transcriptional cascade, including activation of a set of primary response transcription factors. The hierarchical organization of this pathway has left the direct role of EcR in mediating ecdysone responses obscured. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17275 GPL25244

8 Samples

Download data: BW, CSV, NARROWPEAK

(Submitter supplied) In Drosophila, the ecdysone steroid hormone is essential for coordinating developmental timing across physically separated tissues. Ecdysone directly impacts genome function through its receptor, a heterodimer of the EcR and Usp proteins. Ligand binding to EcR triggers a transcriptional cascade, including activation of a set of primary response transcription factors. The hierarchical organization of this pathway has left the direct role of EcR in mediating ecdysone responses obscured. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25244

20 Samples

Download data: BW, CSV, NARROWPEAK

(Submitter supplied) Cells respond to changes in environment by shifting their gene expression profile to deal with the new conditions. The cellular response to changes in metal homeostasis is an important example of this. Transition metals such as iron, zinc, and copper are essential micronutrients but other metals such as cadmium are simply toxic. The cell must maintain metal concentrations in a window that supports efficient metabolic function but must also protect against the damaging effects of high concentrations of these metals. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6629

3 Samples

Download data: BAR, CEL