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Series GSE11752 Query DataSets for GSE11752
Status Public on Sep 21, 2009
Title FRUM and EcR-regulated expression during Drosophila development
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary In Drosophila, male-specific FRU (FRUM) is required to establish the potential for courtship behaviors, but the downstream effectors of FRUM during development are largely unknown. A microarray-based approach identified genes that are differentially expressed as a consequence of FRUM in pupae, in both whole body and CNS tissues. Genes were also identified that are sex-differentially expressed in CNS tissues. The FRUM-regulated sets were significantly overrepresented with genes also regulated by the ecdysone regulatory pathway. Two EcR isoforms (EcRA and EcRB1) are expressed in FRUM-expressing neurons during distinct periods of metamorphosis. Males with abrogated EcRA function in FRUM-expressing neurons aggressively court other males. Transcriptional profiles of mutants with abrogated EcRA function in the fru circuit demonstrate that EcR and FRUM regulate common gene sets, including the early gene broad. These results demonstrate a novel role for EcR in specifying male courtship behavior through its actions specifically in the FRUM neural circuitry.
 
Overall design All microarrays were dual channel with direct comparisons of male versus female, wild type versus mutant, or experimental versus control. For each experiment, four to eight independent biological samples were analyzed using a dye-swap design. Samples consisted of whole body pupae or dissected CNS collected either at 0 hour After Pupal Formation (APF), 48 hour APF, or 0-24 hour adults.
 
Contributor(s) Sanders LE, Lebo MS, Dalton JE, Sun F, Arbeitman MN
Citation(s) 19646872
Submission date Jun 10, 2008
Last update date Mar 19, 2012
Contact name Matthew S Lebo
Organization name University of Southern California
Department Molecular and Copmutational Biology
Lab Arbeitman
Street address 1040 Child's Way
City Los Angeles
State/province CA
ZIP/Postal code 90254
Country USA
 
Platforms (1)
GPL6799 INDAC Drosophila melanogaster 15K v5.0
Samples (30)
GSM297121 fru P1 48 hr APF pupae - whole body - replicate 1
GSM297122 fru P1 48 hr APF pupae - whole body - replicate 2
GSM297123 fru P1 48 hr APF pupae - whole body - replicate 3
Relations
BioProject PRJNA106043

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Supplementary file Size Download File type/resource
GSE11752_RAW.tar 47.8 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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