GEO DataSets

Analysis of PAO1 strain PA2384 null mutant grown under iron-limiting conditions. PA2384 is responsive to iron limitation and is weakly similar to the ferric uptake regulator fur. RNA extracted from cells at various stages of growth. Results provide insight into the function of PA2384.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 genotype/variation, 3 other sets

Platform:

GPL84

Series:

GSE2885

12 Samples

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(Submitter supplied) The gene PA2384 in Pseudomonas aeruginosa PAO1, annotated originally as unknown function, has been previously shown to be dramatically responsive to iron limitation. In the present study, PA2384 is shown by bioinformatics analysis to have a weak similarity to the N-termini DNA binding domain of fur, a well-known ferric uptake regulator. To experimentally investigate the function of PA2384 P. aeruginosa PAO1 recombinant (pUCP20::PA2384) overexpressing PA2384 and PA2384 knock-out mutant PAO1-23844 are constructed and studied. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS1910

Platform:

GPL84

12 Samples

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(Submitter supplied) Virulence factor production and the development of biofilms in Pseudomonas aeruginosa have been shown to be regulated by two hierarchically organised quorum sensing (QS) systems via small acyl-homoserine lactone (AHL) signal molecules. Recently, a third bacterial signal molecule, the Pseudomonas quinolone signal (PQS), has been identified, which positively regulates a subset of genes dependent on the QS systems. more...

Organism:

Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2764

Platform:

GPL84

12 Samples

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Analysis of Pseudomonas aeruginosa cells treated for up to 20 hours with 3,4-(di)hydroxy-heptyl-quinoline (PQS), a bacterial intercellular signaling molecule. Results provide insight into the role of PQS in the bacterial communication system termed quorum sensing.

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array, count, 2 agent, 3 time sets

Platform:

GPL84

Series:

GSE3836

12 Samples

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(Submitter supplied) Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-2), and four non–clonal CF P. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

8 Samples

Download data: CEL, CHP

(Submitter supplied) Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-1), and four non–clonal CF P. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

18 Samples

Download data: CEL, CHP

(Submitter supplied) It has been proposed that the gastro-intestinal tract environment containing high levels of neuroendocrine hormones is important for gut-derived P. aeruginosa infections. In this study, we report that the hormone norepinephrine increases P. aeruginosa PA14 growth, virulence factor production, invasion of HCT-8 epithelial cells, and swimming motility in a concentration-dependent manner. Transcriptome analysis of P. more...

Organism:

Pseudomonas aeruginosa UCBPP-PA14; Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

4 Samples

Download data: CEL

(Submitter supplied) To identify the difference of gene expression in barley upon P. aeruginosa PAO1 and less pathogenic PA5021 mutant Keywords: Disease response

Organism:

Hordeum vulgare

Type:

Expression profiling by array

Platform:

GPL1340

3 Samples

Download data: CEL

(Submitter supplied) Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2870

Platform:

GPL84

20 Samples

Download data: CEL

Analysis of Pseudomonas aeruginosa E601 grown in media containing various sources of sulfur. P. aeruginosa E601 is a cystic fibrosis isolate that exhibits mucin sulfatase activity. Results provide insight into the pathways that respond to the nature of the supply of sulfur.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, transformed count, 5 growth protocol, 2 strain sets

Platform:

GPL84

Series:

GSE8408

20 Samples

Download data: CEL

(Submitter supplied) Anthropogenic pollution has increased the levels of heavy metals in the environment. Bacterial populations continue to thrive in highly polluted environments and bacteria must have mechanisms to counter heavy metal stress. We chose to examine the response of the environmentally-relevant organism Pseudomonas aeruginosa to two different copper treatments. A short, 45 min exposure to copper was done in the Cu shock treatment to examine the immediate transcriptional profile to Cu stress. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2377

Platform:

GPL84

8 Samples

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Analysis of cells subjected to elevated copper (Cu) for 45 minutes (Cu shock) or grown in the presence of elevated Cu for 6 hours (Cu adapted). Industrial activity results in elevated levels of environmental Cu. Results provide insight into the molecular response of P. aeruginosa to Cu stress.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 protocol, 4 stress sets

Platform:

GPL84

Series:

GSE4152

8 Samples

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(Submitter supplied) We performed high-time-resolution (HTR) transcriptome analyses of Pseudomonas aeruginosa PAO1 (PA) in a continuous cultivation system during the transition from high oxygen tension to low oxygen tension (HLOT) and the reversed transition from low to high oxygen tension (LHOT). From those genes responsive to both transient conditions, we identified 85 essential oxygen-availability responsive genes (EORGs), including the expected ones (arcDABC) encoding enzymes for arginine fermentation. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

28 Samples

Download data: CEL

(Submitter supplied) Pseudomonas aeruginosa strain PAO1 was grown at 22°C and 37°C in Lysogeny broth (LB) and RNA was hybridized on the Affymetrix P. aeruginosa chip.

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL, TXT

(Submitter supplied) To gain insights into the initial phases of P. aeruginosa infections and to identify P. aeruginosa genes regulated in response to respiratory epithelia we exposed P. aeruginosa to cultured primary differentiated human airway epithelia. We used a P. aeruginosa strain (PAO1) that causes acute damage to the epithelia and a mutant (PAOSC11) with defects in Type III secretion and in rhamnolipid synthesis. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2502

Platform:

GPL84

8 Samples

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Analysis of Pseudomonas aeruginosa PAO1 or Type III secretion system (TTSS), rhamnolipid synthesis double mutant strain exposed to cultured airway epithelia. TTSS is a virulence factor in acute infections. Strains from chronic infections express decreased levels of TTSS or lack a functional TTSS.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 agent, 2 strain sets

Platform:

GPL84

Series:

GSE4614

8 Samples

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(Submitter supplied) As production of virulence factors by Pseudomonas aeruginosa is influenced by the host environment, we examined the effect of 10% adult bovine serum (ABS) on the global transcription with P. aeruginoas PAO1 at different phases of growth. At early exponential phase (4 h), serum significantly enhanced expression of 138 genes, most of which are repressed by iron and carry binding sequences for the ferric uptake regulator or the Fur-regulated extracytoplasmic function sigma factor PvdS. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL13921

16 Samples

Download data: TXT

(Submitter supplied) Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. We have used Affimetrix microarrays to investigate the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant (PAO6344) under cyanogenic conditions.

Organism:

Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

4 Samples

Download data: CEL, CHP

(Submitter supplied) In the present study, we employed Affymetrix Pseudomonas aeruginosa GeneChip arrays to investigate global gene expression profiles during the cellular response of Pseudomonas aeruginosa to sodium hypochlorite Keywords: Antimicrobial response

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

9 Samples

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(Submitter supplied) The TyrR-like enhancer-binding protein GcsR (or PA2449) was shown to regulate the expression of genes required for glycine metabolism. In order to define the regulon of GcsR we compared the transcriptome of a gcsR deletion mutant of P. aeruginosa PAO1 with that of the wild-type using RNA-Seq.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21297

4 Samples

Download data: TXT