GEO DataSets

Expression profiling of PC12 pheochromocytoma cells treated with 10 uM forskolin for 1 hour. Forskolin activates adenylate cyclase. Effect of 1,9 dideoxy forskolin, an inactive analog of forskolin, also examined. Results identify genes whose expression responds to changes in cAMP levels.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL342

Series:

GSE2071

4 Samples

Download data

(Submitter supplied) PC12 cells (passage 22) were cultured at 5% CO2 in a 37 C incubator. The growth medium was DMEM (high glucose, Invitrogen) supplemented with 25mM HEPES, 10% FCS, 5% FBS and 1x Pen/Strep (Invitrogen). 1x106 cells were starved overnight in DMEM+25 mM HEPES and treated with 10 uM 1,9 dideoxy forskolin or forskolin for 60 min. The final DMSO concentration was 0.05%. Total RNA was purified using Trizol (Invitrogen) according to the manufacturers protocol. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS1038 GDS1039

Platforms:

GPL342 GPL341

8 Samples

Download data

Expression profiling of PC12 pheochromocytoma cells treated with 10 uM forskolin for 1 hour. Forskolin activates adenylate cyclase. Effect of 1,9 dideoxy forskolin, an inactive analog of forskolin, also examined. Results identify genes whose expression responds to changes in cAMP levels.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL341

Series:

GSE2071

4 Samples

Download data

(Submitter supplied) Identifying the chromosomal targets of transcription factors is important for reconstructing the transcriptional regulatory networks underlying global gene expression programs. We have developed an unbiased genomic method called sequence tag analysis of genomic enrichment (STAGE) to identify the direct binding targets of transcription factors in vivo. STAGE is based on high-throughput sequencing of concatemerized tags derived from target DNA enriched by chromatin immunoprecipitation. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL1485

2 Samples

Download data

(Submitter supplied) 2091 mock vs. 2091 E2F4 IP - Human 13K promoter microarray 2091 cells were serum starved for 72hours and fixed with formaldehyde. Keywords: repeat sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1525

2 Samples

Download data

(Submitter supplied) Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL9325

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL11002 GPL10333

16 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Metabolic homeostasis in mammals critically depends on the regulation of fasting-induced genes by CREB in the liver. Previous genome-wide analysis has shown that only a small percentage of CREB target genes are induced in response to fasting-associated signaling pathways. The precise molecular mechanisms by which CREB specifically targets these genes in response to alternating hormonal cues remain to be elucidated. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

4 Samples

Download data: TXT

(Submitter supplied) Metabolic homeostasis in mammals critically depends on the regulation of fasting-induced genes by CREB in the liver. Previous genome-wide analysis has shown that only a small percentage of CREB target genes are induced in response to fasting-associated signaling pathways. The precise molecular mechanisms by which CREB specifically targets these genes in response to alternating hormonal cues remain to be elucidated. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11002

12 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) We report here genome-wide analysis of the tumor suppressor p53 binding sites in normal human cells. 743 high-confidence ChIP-seq peaks representing putative genomic binding sites were identified in normal IMR90 fibroblasts using a reference chromatin sample. More than 40 % were located within 2 kb of a transcription start site (TSS), a distribution similar to that documented for individually studied functional p53 binding sites and to date not observed by previous genome-wide studies. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL10999

2 Samples

Download data: BEDGRAPH, TXT