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SRX7285992: GSM4209493: mC_brain_chicken; Gallus gallus; Bisulfite-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 392M spots, 39.6G bases, 18.1Gb downloads

Submitted by: NCBI (GEO)
Study: Evolution of CpH methylation in vertebrate brains
show Abstracthide Abstract
The brain requires complex mechanisms of genome regulation to encode and store behavioural information. In mammals, DNA methylation deposited at non-CG dinucleotides characterises brain epigenomes. However, it is unclear to what extent this non-canonical form of DNA methylation is evolutionarily conserved. To test this we profile brain cytosine methylation across the major vertebrate lineages, amphioxus, honeybee and octopus, finding that non-CG methylation in adult brain methylomes is restricted to vertebrates. In vertebrates, the genomic patterns of non-CG do not recapitulate those of CG methylation, yet both patterns are deeply conserved. Whereas low levels of gene body CG methylation demarcate a set of developmental transcription factors across tissues and species, a distinct set of neurodevelopmental genes accumulate non-CG methylation in neural tissues. We further show that the establishment of this methylation context coincides with the origin of the “writer” of non-CG methylation, the methyltransferase DNMT3A, and the “reader”, the methyl-CpG binding protein 2 (MeCP2), fuelled by the ancestral whole genome duplication in vertebrates. Surprisingly, MeCP2 evolved in a stepwise process, from an ancestral MBD4 protein with a dual role in transcriptional regulation and DNA repair in chordates. In sum, we show how a novel neural epigenomic layer assembled at the root of vertebrates and gained new regulatory roles partly independent from CG methylation, which could have fostered the sophisticated cognitive repertoires found in the vertebrate lineage. Overall design: Profiling of cytosine methylation for brain samples from Monodelphis domestica, Ornithorhynchus anatinus, Gallus gallus, Callorhinchus milii, Lethenteron camtschaticum, Apis mellifera, Octopus bimaculoides, and Branchiostoma lanceolatum.
Sample: mC_brain_chicken
SAMN13508513 • SRS5779514 • All experiments • All runs
Organism: Gallus gallus
Library:
Instrument: Illumina HiSeq 1500
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: DNeasy Blood & Tissue kit (Qiagen) Genomic DNA was fragmented with a Covaris S2 sonicator to a mean length of 200 bp, then end-repaired, A-tailed, ligated to methylated Illumina TruSeq adapters, bisulfite converted and subjected to 7 cycles of PCR amplification with KAPA HiFi Uracil+ DNA polymerase (KAPA Biosystems).
Experiment attributes:
GEO Accession: GSM4209493
Links:
Runs: 1 run, 392M spots, 39.6G bases, 18.1Gb
Run# of Spots# of BasesSizePublished
SRR10606700392,035,41739.6G18.1Gb2020-10-26

ID:
9562288

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