GSM4061724: A549_AKT1; Homo sapiens; RNA-Seq - SRA

SRX6804713: GSM4061724: A549_AKT1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 47.4M spots, 14.2G bases, 4.1Gb downloads

Submitted by: NCBI (GEO)

Study: Strand specific Sequencing of myr-Akt1 induced transcriptomes in NSCLC cell lines

PRJNA564014 • SRP220432 • All experiments • All runs

show Abstracthide Abstract

To identify the genes significantly regulated by Akt1 activation in NSCLC, myr-Akt1 stable over-expressed A549, HCC827 cells and control vector cells were constructed. The transcriptomes were analyzed by rRNA-depleted strand specific RNA-seq, and LINC01546 was identified significantly up-regulated in myr-Akt1 A549 and HCC827 cells, as compared to vector cells. Overall design: Transcriptomes profiles of A549-myr-Akt1, A549-vector, HCC827-myr-Akt1 and HCC827-vecotr were generated by Illumina NovaSeq 6000.

Sample: A549_AKT1

SAMN12698114 • SRS5349631 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was harvested using Trizol reagent. RNA was prepared by using Ribo-Zero Magnetic Kit (rRNA-depleted RNA). Illumina TruSeq Stranded mRNA Kit was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4061724

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 47.4M spots, 14.2G bases, 4.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR10071126	47,359,483	14.2G	4.1Gb	2019-11-01

ID:: 8963302

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Sequence Read Archive

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