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    brp bruchpilot [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35977, updated on 17-Dec-2024

    Summary

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    Official Symbol
    brpprovided by FlyBase
    Official Full Name
    bruchpilotprovided by FlyBase
    Primary source
    FLYBASE:FBgn0259246
    Locus tag
    Dmel_CG42344
    See related
    AllianceGenome:FB:FBgn0259246
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    Brp; BRP; Bruchpilot; Cast; CG12932; CG12933; CG1931; CG30336; CG30337; CG34146; CG42344; Dmel\CG42344; mAb nc82; mAbnc82; Mabnc82; MAbnc82; nc-82; nc82; Nc82; NC82
    Old locus tag
    Dmel_CG12932; Dmel_CG34146
    Summary
    Predicted to be a structural constituent of presynaptic active zone. Involved in several processes, including chemical synaptic transmission; cytoskeletal matrix organization at active zone; and memory. Located in several cellular components, including cytoskeleton of presynaptic active zone; neuromuscular junction; and presynaptic membrane. Is expressed in several structures, including Kenyon cell; adult brain; adult head; outer photoreceptor cell; and synaptic neuropil domain. Orthologous to human ERC1 (ELKS/RAB6-interacting/CAST family member 1) and ERC2 (ELKS/RAB6-interacting/CAST family member 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See brp in Genome Data Viewer
    Location:
    45E1-45F1; 2-60 cM
    Exon count:
    26
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (9503765..9541565)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (5391270..5429070)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene Calmodulin-binding transcription activator Neighboring gene uncharacterized protein Neighboring gene Wnt oncogene analog 2 Neighboring gene long non-coding RNA:CR43651 Neighboring gene uncharacterized protein Neighboring gene mir-14 stem loop

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Immunostaining of the developing embryonic and larval Drosophila brain. Diaper DC, et al. Methods Mol Biol, 2014. PMID 24048923
    2. Sustaining rapid vesicular release at active zones: potential roles for vesicle tethering. Hallermann S, et al. Trends Neurosci, 2013 Mar. PMID 23164531, Free PMC Article
    3. BRP-170 and BRP190 isoforms of Bruchpilot protein differentially contribute to the frequency of synapses and synaptic circadian plasticity in the visual system of Drosophila. Woźnicka O, et al. Front Cell Neurosci, 2015. PMID 26175667, Free PMC Article
    4. Mapping neural circuits with activity-dependent nuclear import of a transcription factor. Masuyama K, et al. J Neurogenet, 2012 Mar. PMID 22236090, Free PMC Article
    5. Role of GABAergic inhibition in shaping odor-evoked spatiotemporal patterns in the Drosophila antennal lobe. Wilson RI, et al. J Neurosci, 2005 Oct 5. PMID 16207866, Free PMC Article

    See all (664) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix.
      Title: Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix.
    2. localization of BRP-an essential active zone scaffolding protein in Drosophila, depends on the precise balance of neuronal Par-1 kinase.
      Title: Levels of Par-1 kinase determine the localization of Bruchpilot at the Drosophila neuromuscular junction synapses.
    3. Consistent with a block in axonal transport, we find a decrease in number of active zones and reduced neurotransmission in flies overexpressing Par-1 kinase. Interestingly, we find that Par-1 acts independently of Tau-one of the most well studied substrates of Par-1, revealing a presynaptic function for Par-1 that is independent of Tau
      Title: Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase.
    4. The presynaptic protein Brp promotes evoked transmission and suppresses spontaneous release.
      Title: Evoked and spontaneous transmission favored by distinct sets of synapses.
    5. The precise nanoscopic organization of Brp distinguishes different physiological active zones (AZs) and links functional diversification to a heretofore unrecognized neuronal gradient of the AZ cytomatrix ultrastructure.
      Title: Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states.
    6. CK2alpha is a novel regulator of the active zone protein, BRP, in Drosophila.
      Title: CK2α regulates the transcription of BRP in Drosophila.
    7. The macromolecular architecture created by the alternating pattern of the Bruchpilot isoforms determines the number of Ca(2+) channel-coupled SV release slots available per AZ and thereby sets the size of the RRP.
      Title: The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.
    8. This study found a circadian rhythm of BRP expression in the first optic neuropil (lamina) of the optic lobe and in the brain of D. melanogaster. In LD 12:12 the rhythm of BRP abundance is bimodal, displaying morning (ZT1) and evening (ZT13) peaks
      Title: Circadian expression of the presynaptic active zone protein Bruchpilot in the lamina of Drosophila melanogaster.
    9. Review describes how genetic elimination of Bruchpilot revealed that the protein is essential for T-bar formation, calcium channel clustering, and hence proper vesicle fusion and patterned synaptic plasticity.
      Title: The active zone T-bar--a plasticity module?
    10. Bruchpilot, a ubiquitous presynaptic active zone protein, is required for anesthesia-resistant olfactory memory.
      Title: Bruchpilot, a synaptic active zone protein for anesthesia-resistant memory.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables structural constituent of presynaptic active zone IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in adult locomotory behavior IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in anesthesia-resistant memory IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chemical synaptic transmission IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chemical synaptic transmission IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cytoskeletal matrix organization at active zone IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maintenance of presynaptic active zone structure IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in maintenance of presynaptic active zone structure IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neuromuscular synaptic transmission IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neurotransmitter secretion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in presynaptic active zone organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of synaptic plasticity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in short-term memory IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of synapse maturation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in axon terminus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoskeleton of presynaptic active zone IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in neuromuscular junction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in presynaptic active zone IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in presynaptic active zone cytoplasmic component IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in presynaptic membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    bruchpilot
    Names
    CG42344-PD
    CG42344-PE
    CG42344-PG
    CG42344-PH
    CG42344-PI
    CG42344-PJ
    CG42344-PK
    CG42344-PL
    CG42344-PM
    CG42344-PN
    CG42344-PO
    CG42344-PP
    CG42344-PQ
    CG42344-PR
    brp-PD
    brp-PE
    brp-PG
    brp-PH
    brp-PI
    brp-PJ
    brp-PK
    brp-PL
    brp-PM
    brp-PN
    brp-PO
    brp-PP
    brp-PQ
    brp-PR
    brunchpilot
    burchpilot
    monoclonal antibody nc82
    nc82

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      9503765..9541565
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001414094.1 → NP_001400998.1  bruchpilot, isoform N [Drosophila melanogaster]

      Status: REVIEWED

    2. NM_001259299.3 → NP_001246228.1  bruchpilot, isoform J [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246228.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4K7K9
      Conserved Domains (4) summary
      pfam11759
      Location:332 → 401
      KRTAP; Keratin-associated matrix
      TIGR02168
      Location:1509 → 2215
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam01576
      Location:789 → 1824
      Myosin_tail_1; Myosin tail
      pfam09736
      Location:2091 → 2157
      Bud13; Pre-mRNA-splicing factor of RES complex

    3. NM_001414096.1 → NP_001401000.1  bruchpilot, isoform P [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q25B55

    4. NM_001414097.1 → NP_001401001.1  bruchpilot, isoform Q [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q25B55

    5. NM_001414098.1 → NP_001401002.1  bruchpilot, isoform R [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q25B55

    6. NM_001414095.1 → NP_001400999.1  bruchpilot, isoform O [Drosophila melanogaster]

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q25B55

    7. NM_001273897.2 → NP_001260826.1  bruchpilot, isoform M [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260826.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4KF38, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:813 → 1469
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:427 → 1133
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:115 → 663
      Cast; RIM-binding protein of the cytomatrix active zone

    8. NM_165677.4 → NP_724796.3  bruchpilot, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_724796.3

      Status: REVIEWED

      UniProtKB/TrEMBL
      A1Z7V1, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:773 → 1616
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:372 → 1161
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:115 → 695
      Cast; RIM-binding protein of the cytomatrix active zone

    9. NM_001103771.4 → NP_001097241.2  bruchpilot, isoform G [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001097241.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8DY79, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:819 → 1662
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:418 → 1207
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:161 → 741
      Cast; RIM-binding protein of the cytomatrix active zone

    10. NM_001259301.2 → NP_001246230.1  bruchpilot, isoform L [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246230.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4K6V0, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:814 → 1657
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:415 → 1202
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:161 → 736
      Cast; RIM-binding protein of the cytomatrix active zone

    11. NM_001259300.2 → NP_001246229.1  bruchpilot, isoform K [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246229.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4K7V8, Q25B55
      Conserved Domains (3) summary
      TIGR02168
      Location:978 → 1684
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      TIGR02169
      Location:554 → 1296
      SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type
      pfam00261
      Location:487 → 721
      Tropomyosin; Tropomyosin

    12. NM_206063.3 → NP_995785.2  bruchpilot, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_995785.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      A1Z7V1, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:773 → 1616
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:372 → 1161
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:115 → 695
      Cast; RIM-binding protein of the cytomatrix active zone

    13. NM_001043070.1 → NP_001036535.1  bruchpilot, isoform H [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001036535.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A1Z7V1, Q25B55
      Conserved Domains (3) summary
      COG1196
      Location:773 → 1616
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      TIGR02168
      Location:372 → 1161
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:115 → 695
      Cast; RIM-binding protein of the cytomatrix active zone

    14. NM_001259298.2 → NP_001246227.1  bruchpilot, isoform I [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246227.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4K843, Q25B55
      Conserved Domains (2) summary
      TIGR02168
      Location:149 → 926
      SMC_prok_B; chromosome segregation protein SMC, common bacterial type
      pfam10174
      Location:9 → 415
      Cast; RIM-binding protein of the cytomatrix active zone
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4K6V0 A0A0B4K6V0
    A0A0B4K7K9 A0A0B4K7K9
    A0A0B4K7V8 A0A0B4K7V8
    A0A0B4K843 A0A0B4K843
    A0A0B4KF38 A0A0B4KF38
    A1Z7V1 A1Z7V1
    A8DY79 A8DY79
    Q25B55 Q25B55
    Q8MRS3 Q8MRS3

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