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RecName: Full=Type II inositol 3,4-bisphosphate 4-phosphatase; AltName: Full=Inositol polyphosphate 4-phosphatase type II

UniProtKB/Swiss-Prot: Q9QWG5.1

Identical Proteins FASTA Graphics 

LOCUS       INP4B_RAT                928 aa            linear   ROD 27-NOV-2024
DEFINITION  RecName: Full=Type II inositol 3,4-bisphosphate 4-phosphatase;
            AltName: Full=Inositol polyphosphate 4-phosphatase type II.
ACCESSION   Q9QWG5
VERSION     Q9QWG5.1
DBSOURCE    UniProtKB: locus INP4B_RAT, accession Q9QWG5;
            class: standard.
            extra accessions:O35825
            created: Aug 30, 2005.
            sequence updated: May 1, 2000.
            annotation updated: Nov 27, 2024.
            xrefs: U96920.1, AAB72151.1, U96921.1, AAB72152.1, NP_446369.1,
            XP_008770684.1, XP_008770686.1, XP_017456652.1, XP_017456653.1
            xrefs (non-sequence databases): AlphaFoldDB:Q9QWG5, SMR:Q9QWG5,
            STRING:10116.ENSRNOP00000025013, SwissLipids:SLP:000000896,
            iPTMnet:Q9QWG5, PhosphoSitePlus:Q9QWG5,
            PaxDb:10116-ENSRNOP00000025013, Ensembl:ENSRNOT00000024981.7,
            Ensembl:ENSRNOP00000024981.4, Ensembl:ENSRNOG00000018382.7,
            Ensembl:ENSRNOT00000025013.5, Ensembl:ENSRNOP00000025013.4,
            Ensembl:ENSRNOT00055013428, Ensembl:ENSRNOP00055010753,
            Ensembl:ENSRNOG00055007963, Ensembl:ENSRNOT00060020295,
            Ensembl:ENSRNOP00060015957, Ensembl:ENSRNOG00060011966,
            Ensembl:ENSRNOT00065016487, Ensembl:ENSRNOP00065012532,
            Ensembl:ENSRNOG00065010215, GeneID:116699, KEGG:rno:116699,
            AGR:RGD:620470, CTD:8821, RGD:620470, eggNOG:KOG4428,
            GeneTree:ENSGT00940000157587, HOGENOM:CLU_007802_1_0_1,
            InParanoid:Q9QWG5, OMA:VITGRRX, OrthoDB:5490552at2759,
            PhylomeDB:Q9QWG5, TreeFam:TF325637, BRENDA:3.1.3.66,
            Reactome:R-RNO-1660499, Reactome:R-RNO-1660516,
            Reactome:R-RNO-1855183, UniPathway:UPA00944, PRO:PR:Q9QWG5,
            Proteomes:UP000002494, Bgee:ENSRNOG00000018382, GO:0005737,
            GO:0017161, GO:0052828, GO:0008289, GO:0034593, GO:0034594,
            GO:0016316, GO:0006874, GO:0045671, GO:0046856, GO:0046850,
            GO:0046822, GO:0051896, CDD:cd04048, FunFam:2.60.40.150:FF:000143,
            Gene3D:2.60.40.150, InterPro:IPR000008, InterPro:IPR035892,
            InterPro:IPR039034, PANTHER:PTHR12187, PANTHER:PTHR12187:SF3,
            SUPFAM:SSF49562, PROSITE:PS50004
KEYWORDS    Alternative splicing; Hydrolase; Lipid metabolism; Reference
            proteome.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 928)
  AUTHORS   Norris,F.A., Atkins,R.C. and Majerus,P.W.
  TITLE     The cDNA cloning and characterization of inositol polyphosphate
            4-phosphatase type II. Evidence for conserved alternative splicing
            in the 4-phosphatase family
  JOURNAL   J Biol Chem 272 (38), 23859-23864 (1997)
   PUBMED   9295334
  REMARK    NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CATALYTIC ACTIVITY,
            ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND
            FUNCTION.;
            TISSUE=Brain
COMMENT     On Mar 21, 2006 this sequence version replaced gi:81861214.
            [FUNCTION] Catalyzes the hydrolysis of the 4-position phosphate of
            phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate
            and inositol 3,4-bisphosphate (PubMed:9295334). Plays a role in the
            late stages of macropinocytosis by dephosphorylating
            phosphatidylinositol 3,4-bisphosphate in membrane ruffles (By
            similarity). The lipid phosphatase activity is critical for tumor
            suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway
            by dephosphorylating phosphoinositides and thereby modulating cell
            cycle progression and cell survival (By similarity).
            {ECO:0000250|UniProtKB:O15327, ECO:0000269|PubMed:9295334}.
            [CATALYTIC ACTIVITY] Reaction=a
            1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate)
            + H2O = a
            1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) +
            phosphate; Xref=Rhea:RHEA:17193, ChEBI:CHEBI:15377,
            ChEBI:CHEBI:43474, ChEBI:CHEBI:57658, ChEBI:CHEBI:58088;
            EC=3.1.3.66; Evidence={ECO:0000269|PubMed:9295334}.
            [CATALYTIC ACTIVITY] Reaction=1D-myo-inositol 3,4-bisphosphate +
            H2O = 1D-myo-inositol 3-phosphate + phosphate;
            Xref=Rhea:RHEA:43388, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
            ChEBI:CHEBI:58401, ChEBI:CHEBI:83241;
            Evidence={ECO:0000269|PubMed:9295334}.
            [CATALYTIC ACTIVITY] Reaction=1D-myo-inositol 1,3,4-trisphosphate +
            H2O = 1D-myo-inositol 1,3-bisphosphate + phosphate;
            Xref=Rhea:RHEA:43392, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
            ChEBI:CHEBI:58414, ChEBI:CHEBI:83242;
            Evidence={ECO:0000269|PubMed:9295334}.
            ACTIVITY REGULATION: Strongly inhibited by inositol
            hexakisphosphate. {ECO:0000269|PubMed:9295334}.
            [BIOPHYSICOCHEMICAL PROPERTIES] Kinetic parameters: KM=39 uM for
            inositol 3,4-bisphosphate {ECO:0000269|PubMed:9295334}; KM=34 uM
            for inositol 1,3,4-trisphosphate {ECO:0000269|PubMed:9295334};
            Vmax=26 umol/min/mg enzyme with inositol 3,4-bisphosphate as
            substrate {ECO:0000269|PubMed:9295334}; Vmax=22 umol/min/mg enzyme
            with inositol 1,3,4-trisphosphate as substrate
            {ECO:0000269|PubMed:9295334}; pH dependence: Optimum pH is 7-8.
            {ECO:0000269|PubMed:9295334}.
            [PATHWAY] Signal transduction; phosphatidylinositol signaling
            pathway. {ECO:0000269|PubMed:9295334}.
            [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named
            isoforms=2; Name=1; Synonyms=Alpha; IsoId=Q9QWG5-1;
            Sequence=Displayed; Name=2; Synonyms=Beta; IsoId=Q9QWG5-2;
            Sequence=VSP_015249.
            [MISCELLANEOUS] [Isoform 2]: Inactive. {ECO:0000305}.
            [SIMILARITY] Belongs to the inositol 3,4-bisphosphate 4-phosphatase
            family. {ECO:0000305}.
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
     gene            1..928
                     /gene="Inpp4b"
     Protein         1..928
                     /product="Type II inositol 3,4-bisphosphate 4-phosphatase"
                     /EC_number="3.1.3.66"
                     /note="Inositol polyphosphate 4-phosphatase type II"
                     /UniProtKB_evidence="Evidence at protein level"
     Region          1..928
                     /region_name="Mature chain"
                     /note="Type II inositol 3,4-bisphosphate 4-phosphatase.
                     /id=PRO_0000190239."
     Region          1..23
                     /region_name="Region of interest in the sequence"
                     /note="Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite."
     Region          23..165
                     /region_name="Domain"
                     /note="C2.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00041."
     Region          39..175
                     /region_name="C2A_Copine"
                     /note="C2 domain first repeat in Copine; cd04048"
                     /db_xref="CDD:176013"
     Site            order(54,60,116,118,143)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176013"
     Region          481..516
                     /region_name="Region of interest in the sequence"
                     /note="Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite."
     Region          488..516
                     /region_name="Compositionally biased region"
                     /note="Basic and acidic residues.
                     /evidence=ECO:0000256|SAM:MobiDB-lite."
     Region          548..575
                     /region_name="Region of interest in the sequence"
                     /note="Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite."
     Region          885..928
                     /region_name="Splicing variant"
                     /note="REGCRIENVLKNIKCRRYAFNMLQLMAFPKCYRPPEGTYGKADT ->
                     SRQTQGALNESDDPETGCLSDNKPTSRHFYPVALLLVSSHLLVVWLILSLALLLAKYQ
                     (in isoform 2). /evidence=ECO:0000303|PubMed:9295334.
                     /id=VSP_015249."
ORIGIN      
        1 meikeegtse egqhflpaaq andpediqft siqkspnepq lefilackdl vapvsdrkln
       61 tvvqvsvihp veqtltryss teivegtkdp lfltgvtfps dypiyeetri kltvydvkdk
      121 phdtirtsvl pehkdpppev arsflgcasf kvgellkske qllslslrts dggkvvgtie
      181 vslvkmgeie dgdtdhittd vqgqkcalvy dstapeslsg kenlpfmnav lrnpvcklyr
      241 fptsdnkwmr ireqmsesil sfhipkelis lhikedlcrn qelkelgdls phwdnlrnnv
      301 lshcdqmvtm yqdiltelsk etgssfksss skgektlefv pinlhlqrmq vhsphlkdal
      361 ydvitvgapa ahfqgfkngg lrkllhrfet errntgyqfi yyspentaka kevlssinql
      421 qplvathadl lltsasqhsp dslrsslkll sektelfvha fkdqlvrsal lalytarpgg
      481 ilrkppspkv steekssqhd spqqlrrqds iphhsdydee ewdrvwanvg kslnciiakv
      541 dklierdsrn dkstggdssk dgdadpnled sltshpredw yeqlhplilt lkecmaevvn
      601 rakqsltfvl lqelayslpq clmltlrrdi vfsqalaglv cgfiiklhts lhdpgflqql
      661 htvglivqye gllstysdei gmledmavgi sdlrkvafki teatsndvlp vltgrrehyv
      721 vevklpatvf eslplqikeg qllhvypvlf nvgineqqtl aerfgdvslq esinqenfel
      781 vqeyysifme kmppdyishf qeqndlkgll dnlhqniqak krknveimwl aaticrklng
      841 irftccksak drtsmsvtle qcsilrdehq lhkdffiral dcmrregcri envlknikcr
      901 ryafnmlqlm afpkcyrppe gtygkadt
//
1..928
/gene="Inpp4b"
Feature Q9QWG5 : 1 segment
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