LOCUS S6A15_RAT 729 aa linear ROD 24-JUL-2024
DEFINITION RecName: Full=Sodium-dependent neutral amino acid transporter
B(0)AT2; AltName: Full=Solute carrier family 6 member 15; AltName:
Full=Transporter v7-3; AltName: Full=sodium- and chloride-dependent
neurotransmitter transporter NTT73.
ACCESSION Q08469
VERSION Q08469.1
DBSOURCE UniProtKB: locus S6A15_RAT, accession Q08469;
class: standard.
extra accessions:Q63838
created: Feb 1, 1995.
sequence updated: Feb 1, 1995.
annotation updated: Jul 24, 2024.
xrefs: L22022.1, AAA41729.1, S56968.1, AAB25532.1, I65413,
NP_758824.1
xrefs (non-sequence databases): AlphaFoldDB:Q08469, SMR:Q08469,
STRING:10116.ENSRNOP00000073005, GlyCosmos:Q08469, GlyGen:Q08469,
iPTMnet:Q08469, PhosphoSitePlus:Q08469,
PaxDb:10116-ENSRNOP00000032996, Ensembl:ENSRNOT00000029208.6,
Ensembl:ENSRNOP00000032996.4, Ensembl:ENSRNOG00000027468.6,
Ensembl:ENSRNOT00055028317, Ensembl:ENSRNOP00055022798,
Ensembl:ENSRNOG00055016667, Ensembl:ENSRNOT00060007903,
Ensembl:ENSRNOP00060005942, Ensembl:ENSRNOG00060004728,
Ensembl:ENSRNOT00065004463, Ensembl:ENSRNOP00065003187,
Ensembl:ENSRNOG00065003168, GeneID:282712, KEGG:rno:282712,
UCSC:RGD:628664, AGR:RGD:628664, CTD:55117, RGD:628664,
eggNOG:KOG3659, GeneTree:ENSGT00940000157277,
HOGENOM:CLU_006855_7_1_1, InParanoid:Q08469, OMA:VVFCLRY,
OrthoDB:3084493at2759, PhylomeDB:Q08469, TreeFam:TF352709,
Reactome:R-RNO-352230, Reactome:R-RNO-442660, PRO:PR:Q08469,
Proteomes:UP000002494, Bgee:ENSRNOG00000027468, GO:0016020,
GO:0005886, GO:0015657, GO:0005295, GO:0005298, GO:0015820,
GO:0006836, GO:0015804, GO:0015824, GO:0035725, CDD:cd11522,
InterPro:IPR042934, InterPro:IPR000175, InterPro:IPR002438,
InterPro:IPR037272, NCBIfam:NF037979, PANTHER:PTHR11616:SF101,
PANTHER:PTHR11616, Pfam:PF00209, PRINTS:PR00176, PRINTS:PR01206,
SUPFAM:SSF161070, PROSITE:PS00610, PROSITE:PS00754, PROSITE:PS50267
KEYWORDS Amino-acid transport; Glycoprotein; Ion transport; Membrane;
Neurotransmitter transport; Phosphoprotein; Reference proteome;
Sodium; Sodium transport; Symport; Transmembrane; Transmembrane
helix; Transport.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (residues 1 to 729)
AUTHORS Uhl,G.R., Kitayama,S., Gregor,P., Nanthakumar,E., Persico,A. and
Shimada,S.
TITLE Neurotransmitter transporter family cDNAs in a rat midbrain
library: 'orphan transporters' suggest sizable structural
variations
JOURNAL Brain Res Mol Brain Res 16 (3-4), 353-359 (1992)
PUBMED 1363329
REMARK NUCLEOTIDE SEQUENCE [MRNA].;
TISSUE=Brain
REFERENCE 2 (residues 1 to 729)
AUTHORS Inoue,K., Sato,K., Tohyama,M., Shimada,S. and Uhl,G.R.
TITLE Widespread brain distribution of mRNA encoding the orphan
neurotransmitter transporter v7-3
JOURNAL Brain Res Mol Brain Res 37 (1-2), 217-223 (1996)
PUBMED 8738154
REMARK TISSUE SPECIFICITY.
REFERENCE 3 (residues 1 to 729)
AUTHORS Lundby,A., Secher,A., Lage,K., Nordsborg,N.B., Dmytriyev,A.,
Lundby,C. and Olsen,J.V.
TITLE Quantitative maps of protein phosphorylation sites across 14
different rat organs and tissues
JOURNAL Nat Commun 3, 876 (2012)
PUBMED 22673903
REMARK PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-699 AND SER-701, AND
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Publication Status: Online-Only
COMMENT On Jul 18, 2007 this sequence version replaced gi:2143978.
[FUNCTION] Functions as a sodium-dependent neutral amino acid
transporter. Exhibits preference for the branched-chain amino
acids, particularly leucine, valine and isoleucine and methionine.
Can also transport low-affinity substrates such as alanine,
phenylalanine, glutamine and pipecolic acid. Mediates the
saturable, pH-sensitive and electrogenic cotransport of proline and
sodium ions with a stoichiometry of 1:1. May have a role as
transporter for neurotransmitter precursors into neurons. In
contrast to other members of the neurotransmitter transporter
family, does not appear to be chloride-dependent.
{ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-leucine(in) + Na(+)(in) =
L-leucine(out) + Na(+)(out); Xref=Rhea:RHEA:29263,
ChEBI:CHEBI:29101, ChEBI:CHEBI:57427;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-isoleucine(in) + Na(+)(in) =
L-isoleucine(out) + Na(+)(out); Xref=Rhea:RHEA:29275,
ChEBI:CHEBI:29101, ChEBI:CHEBI:58045;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-methionine(in) + Na(+)(in) =
L-methionine(out) + Na(+)(out); Xref=Rhea:RHEA:68240,
ChEBI:CHEBI:29101, ChEBI:CHEBI:57844;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-proline(in) + Na(+)(in) =
L-proline(out) + Na(+)(out); Xref=Rhea:RHEA:28967,
ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-alanine(in) + Na(+)(in) =
L-alanine(out) + Na(+)(out); Xref=Rhea:RHEA:29283,
ChEBI:CHEBI:29101, ChEBI:CHEBI:57972;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-asparagine(in) + Na(+)(in) =
L-asparagine(out) + Na(+)(out); Xref=Rhea:RHEA:71383,
ChEBI:CHEBI:29101, ChEBI:CHEBI:58048;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-valine(in) + Na(+)(in) =
L-valine(out) + Na(+)(out); Xref=Rhea:RHEA:29267,
ChEBI:CHEBI:29101, ChEBI:CHEBI:57762;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-cysteine(in) + Na(+)(in) =
L-cysteine(out) + Na(+)(out); Xref=Rhea:RHEA:68232,
ChEBI:CHEBI:29101, ChEBI:CHEBI:35235;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-glutamine(in) + Na(+)(in) =
L-glutamine(out) + Na(+)(out); Xref=Rhea:RHEA:68236,
ChEBI:CHEBI:29101, ChEBI:CHEBI:58359;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-serine(in) + Na(+)(in) =
L-serine(out) + Na(+)(out); Xref=Rhea:RHEA:29575,
ChEBI:CHEBI:29101, ChEBI:CHEBI:33384;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-threonine(in) + Na(+)(in) =
L-threonine(out) + Na(+)(out); Xref=Rhea:RHEA:69999,
ChEBI:CHEBI:29101, ChEBI:CHEBI:57926;
Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
[CATALYTIC ACTIVITY] Reaction=L-pipecolate(in) + Na(+)(in) =
L-pipecolate(out) + Na(+)(out); Xref=Rhea:RHEA:71387,
ChEBI:CHEBI:29101, ChEBI:CHEBI:61185;
Evidence={ECO:0000250|UniProtKB:Q8BG16}.
[CATALYTIC ACTIVITY] Reaction=L-phenylalanine(in) + Na(+)(in) =
L-phenylalanine(out) + Na(+)(out); Xref=Rhea:RHEA:68244,
ChEBI:CHEBI:29101, ChEBI:CHEBI:58095;
Evidence={ECO:0000250|UniProtKB:Q8BG16}.
[SUBCELLULAR LOCATION] Membrane {ECO:0000250|UniProtKB:Q9H2J7};
Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9H2J7}.
[TISSUE SPECIFICITY] Widely distributed in the central nervous
system, including the olfactory bulb, the hypothalamus, the
cerebral cortex, the hippocampus, and the cerebellum. In addition,
intense expression is found in the motor nuclei including the
oculomotor nucleus, abducens nucleus, trigeminal motor nucleus,
facial nucleus, hypoglossal nucleus and ventral horn of spinal
cord. Intense hybridization signals are also observed in the nuclei
containing monoaminergic neurons, such as locus coeruleus, the
substantia nigra pars compacta, the ventral tegmental area, the
dorsal raphe nucleus and the median raphe nucleus.
{ECO:0000269|PubMed:8738154}.
[SIMILARITY] Belongs to the sodium:neurotransmitter symporter (SNF)
(TC 2.A.22) family. SLC6A15 subfamily. {ECO:0000305}.
FEATURES Location/Qualifiers
source 1..729
/organism="Rattus norvegicus"
/db_xref="taxon:10116"
gene 1..729
/gene="Slc6a15"
/gene_synonym="Ntt73"
Protein 1..729
/product="Sodium-dependent neutral amino acid transporter
B(0)AT2"
/note="Solute carrier family 6 member 15; Transporter
v7-3; sodium- and chloride-dependent neurotransmitter
transporter NTT73"
/UniProtKB_evidence="Evidence at protein level"
Region 1..729
/region_name="Mature chain"
/note="Sodium-dependent neutral amino acid transporter
B(0)AT2. /id=PRO_0000214800."
Region 1..69
/region_name="Topological domain"
/note="Cytoplasmic. /evidence=ECO:0000255."
Region 1..3
/region_name="Conflict"
/note="MPK -> IP (in Ref. 1; AAB25532).
/evidence=ECO:0000305."
Site 25
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q9H2J7."
Site 55
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q9H2J7."
Region 61..640
/region_name="SLC6sbd_SBAT1"
/note="Sodium-coupled branched-chain amino-acid
transporter 1; solute-binding domain; cd11522"
/db_xref="CDD:212091"
Region 70..90
/region_name="Transmembrane region"
/note="Helical; Name=1. /evidence=ECO:0000255."
Site order(76,79,470,473..474)
/site_type="other"
/note="Na binding site 2 [ion binding]"
/db_xref="CDD:212091"
Site order(77..78,80..83,158,308..309,314,316,474,477..478)
/site_type="other"
/note="putative substrate binding site 1 [chemical
binding]"
/db_xref="CDD:212091"
Site order(78,83,309,341)
/site_type="other"
/note="Na binding site 1 [ion binding]"
/db_xref="CDD:212091"
Site order(85..86,157,161,164,439,443)
/site_type="other"
/note="putative substrate binding site 2 [chemical
binding]"
/db_xref="CDD:212091"
Region 98..117
/region_name="Transmembrane region"
/note="Helical; Name=2. /evidence=ECO:0000255."
Region 142..162
/region_name="Transmembrane region"
/note="Helical; Name=3. /evidence=ECO:0000255."
Region 163..225
/region_name="Topological domain"
/note="Extracellular. /evidence=ECO:0000255."
Site 187
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255."
Region 226..244
/region_name="Transmembrane region"
/note="Helical; Name=4. /evidence=ECO:0000255."
Region 253..270
/region_name="Transmembrane region"
/note="Helical; Name=5. /evidence=ECO:0000255."
Region 306..323
/region_name="Transmembrane region"
/note="Helical; Name=6. /evidence=ECO:0000255."
Region 335..356
/region_name="Transmembrane region"
/note="Helical; Name=7. /evidence=ECO:0000255."
Region 357..452
/region_name="Topological domain"
/note="Extracellular. /evidence=ECO:0000255."
Site 383
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255."
Site 394
/site_type="glycosylation"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255."
Region 453..472
/region_name="Transmembrane region"
/note="Helical; Name=8. /evidence=ECO:0000255."
Region 496..514
/region_name="Transmembrane region"
/note="Helical; Name=9. /evidence=ECO:0000255."
Region 530..550
/region_name="Transmembrane region"
/note="Helical; Name=10. /evidence=ECO:0000255."
Region 571..592
/region_name="Transmembrane region"
/note="Helical; Name=11. /evidence=ECO:0000255."
Region 620..642
/region_name="Transmembrane region"
/note="Helical; Name=12. /evidence=ECO:0000255."
Region 643..729
/region_name="Topological domain"
/note="Cytoplasmic. /evidence=ECO:0000255."
Site 687
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q9H2J7."
Site 699
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:22673903."
Site 701
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0007744|PubMed:22673903."
ORIGIN
1 mpknskvvkr dldddviesv kdllsnedsv edvskkseli vdvqeekdtd aedgsevdde
61 rpawnsklqy ilaqvgfsvg lgnvwrfpyl cqkngggayl lpylilllvi giplfflels
121 vgqrirrgsi gvwnyispkl ggigfascvv cyfvalyynv iigwtlfyfs qsfqqplpwd
181 qcplvknash tyiepeceks sattyywyre alaisssise sgglnwkmtg cllaawvmvc
241 lamikgiqss gkimyfsslf pyvvlicfli rslllngsid girhmftpkl emmlepkvwr
301 eaatqvffal glgfggviaf ssynkrdnnc hfdavlvsfi nfftsvlatl vvfavlgfka
361 nivnekcisq nsemilkllk tgnvswdvip rhinlsavta edyhvvydii qkvkeeefav
421 lhlkacqied elnkavqgtg lafiafteam thfpaspfws vmfflmlinl glgsmfgtie
481 giitpvvdtf kvrkeiltvi ccllafcigl mfvqrsgnyf vtmfddysat lpllivvile
541 niavsfvygi dkfledltdm lgfapskyyy ymwkyisplm lvtlliasiv nmglsppgyn
601 awikekasee flsypmwgmv vcfslmvlai lpvpvvfvir rcnliddssg nlasvtykrg
661 rvlkepvnld gddaslihgk ipsemsspnf gkniyrkqsg sptldtapng rygigylmad
721 mpdmpesdl
//