U.S. flag

An official website of the United States government

RecName: Full=Sodium-dependent neutral amino acid transporter B(0)AT2; AltName: Full=Solute carrier family 6 member 15; AltName: Full=Transporter v7-3; AltName: Full=sodium- and chloride-dependent neurotransmitter transporter NTT73

UniProtKB/Swiss-Prot: Q08469.1

Identical Proteins FASTA Graphics 

LOCUS       S6A15_RAT                729 aa            linear   ROD 24-JUL-2024
DEFINITION  RecName: Full=Sodium-dependent neutral amino acid transporter
            B(0)AT2; AltName: Full=Solute carrier family 6 member 15; AltName:
            Full=Transporter v7-3; AltName: Full=sodium- and chloride-dependent
            neurotransmitter transporter NTT73.
ACCESSION   Q08469
VERSION     Q08469.1
DBSOURCE    UniProtKB: locus S6A15_RAT, accession Q08469;
            class: standard.
            extra accessions:Q63838
            created: Feb 1, 1995.
            sequence updated: Feb 1, 1995.
            annotation updated: Jul 24, 2024.
            xrefs: L22022.1, AAA41729.1, S56968.1, AAB25532.1, I65413,
            NP_758824.1
            xrefs (non-sequence databases): AlphaFoldDB:Q08469, SMR:Q08469,
            STRING:10116.ENSRNOP00000073005, GlyCosmos:Q08469, GlyGen:Q08469,
            iPTMnet:Q08469, PhosphoSitePlus:Q08469,
            PaxDb:10116-ENSRNOP00000032996, Ensembl:ENSRNOT00000029208.6,
            Ensembl:ENSRNOP00000032996.4, Ensembl:ENSRNOG00000027468.6,
            Ensembl:ENSRNOT00055028317, Ensembl:ENSRNOP00055022798,
            Ensembl:ENSRNOG00055016667, Ensembl:ENSRNOT00060007903,
            Ensembl:ENSRNOP00060005942, Ensembl:ENSRNOG00060004728,
            Ensembl:ENSRNOT00065004463, Ensembl:ENSRNOP00065003187,
            Ensembl:ENSRNOG00065003168, GeneID:282712, KEGG:rno:282712,
            UCSC:RGD:628664, AGR:RGD:628664, CTD:55117, RGD:628664,
            eggNOG:KOG3659, GeneTree:ENSGT00940000157277,
            HOGENOM:CLU_006855_7_1_1, InParanoid:Q08469, OMA:VVFCLRY,
            OrthoDB:3084493at2759, PhylomeDB:Q08469, TreeFam:TF352709,
            Reactome:R-RNO-352230, Reactome:R-RNO-442660, PRO:PR:Q08469,
            Proteomes:UP000002494, Bgee:ENSRNOG00000027468, GO:0016020,
            GO:0005886, GO:0015657, GO:0005295, GO:0005298, GO:0015820,
            GO:0006836, GO:0015804, GO:0015824, GO:0035725, CDD:cd11522,
            InterPro:IPR042934, InterPro:IPR000175, InterPro:IPR002438,
            InterPro:IPR037272, NCBIfam:NF037979, PANTHER:PTHR11616:SF101,
            PANTHER:PTHR11616, Pfam:PF00209, PRINTS:PR00176, PRINTS:PR01206,
            SUPFAM:SSF161070, PROSITE:PS00610, PROSITE:PS00754, PROSITE:PS50267
KEYWORDS    Amino-acid transport; Glycoprotein; Ion transport; Membrane;
            Neurotransmitter transport; Phosphoprotein; Reference proteome;
            Sodium; Sodium transport; Symport; Transmembrane; Transmembrane
            helix; Transport.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 729)
  AUTHORS   Uhl,G.R., Kitayama,S., Gregor,P., Nanthakumar,E., Persico,A. and
            Shimada,S.
  TITLE     Neurotransmitter transporter family cDNAs in a rat midbrain
            library: 'orphan transporters' suggest sizable structural
            variations
  JOURNAL   Brain Res Mol Brain Res 16 (3-4), 353-359 (1992)
   PUBMED   1363329
  REMARK    NUCLEOTIDE SEQUENCE [MRNA].;
            TISSUE=Brain
REFERENCE   2  (residues 1 to 729)
  AUTHORS   Inoue,K., Sato,K., Tohyama,M., Shimada,S. and Uhl,G.R.
  TITLE     Widespread brain distribution of mRNA encoding the orphan
            neurotransmitter transporter v7-3
  JOURNAL   Brain Res Mol Brain Res 37 (1-2), 217-223 (1996)
   PUBMED   8738154
  REMARK    TISSUE SPECIFICITY.
REFERENCE   3  (residues 1 to 729)
  AUTHORS   Lundby,A., Secher,A., Lage,K., Nordsborg,N.B., Dmytriyev,A.,
            Lundby,C. and Olsen,J.V.
  TITLE     Quantitative maps of protein phosphorylation sites across 14
            different rat organs and tissues
  JOURNAL   Nat Commun 3, 876 (2012)
   PUBMED   22673903
  REMARK    PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-699 AND SER-701, AND
            IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
            Publication Status: Online-Only
COMMENT     On Jul 18, 2007 this sequence version replaced gi:2143978.
            [FUNCTION] Functions as a sodium-dependent neutral amino acid
            transporter. Exhibits preference for the branched-chain amino
            acids, particularly leucine, valine and isoleucine and methionine.
            Can also transport low-affinity substrates such as alanine,
            phenylalanine, glutamine and pipecolic acid. Mediates the
            saturable, pH-sensitive and electrogenic cotransport of proline and
            sodium ions with a stoichiometry of 1:1. May have a role as
            transporter for neurotransmitter precursors into neurons. In
            contrast to other members of the neurotransmitter transporter
            family, does not appear to be chloride-dependent.
            {ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-leucine(in) + Na(+)(in) =
            L-leucine(out) + Na(+)(out); Xref=Rhea:RHEA:29263,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:57427;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-isoleucine(in) + Na(+)(in) =
            L-isoleucine(out) + Na(+)(out); Xref=Rhea:RHEA:29275,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:58045;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-methionine(in) + Na(+)(in) =
            L-methionine(out) + Na(+)(out); Xref=Rhea:RHEA:68240,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:57844;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-proline(in) + Na(+)(in) =
            L-proline(out) + Na(+)(out); Xref=Rhea:RHEA:28967,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-alanine(in) + Na(+)(in) =
            L-alanine(out) + Na(+)(out); Xref=Rhea:RHEA:29283,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:57972;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-asparagine(in) + Na(+)(in) =
            L-asparagine(out) + Na(+)(out); Xref=Rhea:RHEA:71383,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:58048;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-valine(in) + Na(+)(in) =
            L-valine(out) + Na(+)(out); Xref=Rhea:RHEA:29267,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:57762;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-cysteine(in) + Na(+)(in) =
            L-cysteine(out) + Na(+)(out); Xref=Rhea:RHEA:68232,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:35235;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-glutamine(in) + Na(+)(in) =
            L-glutamine(out) + Na(+)(out); Xref=Rhea:RHEA:68236,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:58359;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-serine(in) + Na(+)(in) =
            L-serine(out) + Na(+)(out); Xref=Rhea:RHEA:29575,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:33384;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-threonine(in) + Na(+)(in) =
            L-threonine(out) + Na(+)(out); Xref=Rhea:RHEA:69999,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:57926;
            Evidence={ECO:0000250|UniProtKB:Q9H2J7}.
            [CATALYTIC ACTIVITY] Reaction=L-pipecolate(in) + Na(+)(in) =
            L-pipecolate(out) + Na(+)(out); Xref=Rhea:RHEA:71387,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:61185;
            Evidence={ECO:0000250|UniProtKB:Q8BG16}.
            [CATALYTIC ACTIVITY] Reaction=L-phenylalanine(in) + Na(+)(in) =
            L-phenylalanine(out) + Na(+)(out); Xref=Rhea:RHEA:68244,
            ChEBI:CHEBI:29101, ChEBI:CHEBI:58095;
            Evidence={ECO:0000250|UniProtKB:Q8BG16}.
            [SUBCELLULAR LOCATION] Membrane {ECO:0000250|UniProtKB:Q9H2J7};
            Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9H2J7}.
            [TISSUE SPECIFICITY] Widely distributed in the central nervous
            system, including the olfactory bulb, the hypothalamus, the
            cerebral cortex, the hippocampus, and the cerebellum. In addition,
            intense expression is found in the motor nuclei including the
            oculomotor nucleus, abducens nucleus, trigeminal motor nucleus,
            facial nucleus, hypoglossal nucleus and ventral horn of spinal
            cord. Intense hybridization signals are also observed in the nuclei
            containing monoaminergic neurons, such as locus coeruleus, the
            substantia nigra pars compacta, the ventral tegmental area, the
            dorsal raphe nucleus and the median raphe nucleus.
            {ECO:0000269|PubMed:8738154}.
            [SIMILARITY] Belongs to the sodium:neurotransmitter symporter (SNF)
            (TC 2.A.22) family. SLC6A15 subfamily. {ECO:0000305}.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
     gene            1..729
                     /gene="Slc6a15"
                     /gene_synonym="Ntt73"
     Protein         1..729
                     /product="Sodium-dependent neutral amino acid transporter
                     B(0)AT2"
                     /note="Solute carrier family 6 member 15; Transporter
                     v7-3; sodium- and chloride-dependent neurotransmitter
                     transporter NTT73"
                     /UniProtKB_evidence="Evidence at protein level"
     Region          1..729
                     /region_name="Mature chain"
                     /note="Sodium-dependent neutral amino acid transporter
                     B(0)AT2. /id=PRO_0000214800."
     Region          1..69
                     /region_name="Topological domain"
                     /note="Cytoplasmic. /evidence=ECO:0000255."
     Region          1..3
                     /region_name="Conflict"
                     /note="MPK -> IP (in Ref. 1; AAB25532).
                     /evidence=ECO:0000305."
     Site            25
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H2J7."
     Site            55
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H2J7."
     Region          61..640
                     /region_name="SLC6sbd_SBAT1"
                     /note="Sodium-coupled branched-chain amino-acid
                     transporter 1; solute-binding domain; cd11522"
                     /db_xref="CDD:212091"
     Region          70..90
                     /region_name="Transmembrane region"
                     /note="Helical; Name=1. /evidence=ECO:0000255."
     Site            order(76,79,470,473..474)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:212091"
     Site            order(77..78,80..83,158,308..309,314,316,474,477..478)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:212091"
     Site            order(78,83,309,341)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:212091"
     Site            order(85..86,157,161,164,439,443)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:212091"
     Region          98..117
                     /region_name="Transmembrane region"
                     /note="Helical; Name=2. /evidence=ECO:0000255."
     Region          142..162
                     /region_name="Transmembrane region"
                     /note="Helical; Name=3. /evidence=ECO:0000255."
     Region          163..225
                     /region_name="Topological domain"
                     /note="Extracellular. /evidence=ECO:0000255."
     Site            187
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255."
     Region          226..244
                     /region_name="Transmembrane region"
                     /note="Helical; Name=4. /evidence=ECO:0000255."
     Region          253..270
                     /region_name="Transmembrane region"
                     /note="Helical; Name=5. /evidence=ECO:0000255."
     Region          306..323
                     /region_name="Transmembrane region"
                     /note="Helical; Name=6. /evidence=ECO:0000255."
     Region          335..356
                     /region_name="Transmembrane region"
                     /note="Helical; Name=7. /evidence=ECO:0000255."
     Region          357..452
                     /region_name="Topological domain"
                     /note="Extracellular. /evidence=ECO:0000255."
     Site            383
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255."
     Site            394
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255."
     Region          453..472
                     /region_name="Transmembrane region"
                     /note="Helical; Name=8. /evidence=ECO:0000255."
     Region          496..514
                     /region_name="Transmembrane region"
                     /note="Helical; Name=9. /evidence=ECO:0000255."
     Region          530..550
                     /region_name="Transmembrane region"
                     /note="Helical; Name=10. /evidence=ECO:0000255."
     Region          571..592
                     /region_name="Transmembrane region"
                     /note="Helical; Name=11. /evidence=ECO:0000255."
     Region          620..642
                     /region_name="Transmembrane region"
                     /note="Helical; Name=12. /evidence=ECO:0000255."
     Region          643..729
                     /region_name="Topological domain"
                     /note="Cytoplasmic. /evidence=ECO:0000255."
     Site            687
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H2J7."
     Site            699
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903."
     Site            701
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903."
ORIGIN      
        1 mpknskvvkr dldddviesv kdllsnedsv edvskkseli vdvqeekdtd aedgsevdde
       61 rpawnsklqy ilaqvgfsvg lgnvwrfpyl cqkngggayl lpylilllvi giplfflels
      121 vgqrirrgsi gvwnyispkl ggigfascvv cyfvalyynv iigwtlfyfs qsfqqplpwd
      181 qcplvknash tyiepeceks sattyywyre alaisssise sgglnwkmtg cllaawvmvc
      241 lamikgiqss gkimyfsslf pyvvlicfli rslllngsid girhmftpkl emmlepkvwr
      301 eaatqvffal glgfggviaf ssynkrdnnc hfdavlvsfi nfftsvlatl vvfavlgfka
      361 nivnekcisq nsemilkllk tgnvswdvip rhinlsavta edyhvvydii qkvkeeefav
      421 lhlkacqied elnkavqgtg lafiafteam thfpaspfws vmfflmlinl glgsmfgtie
      481 giitpvvdtf kvrkeiltvi ccllafcigl mfvqrsgnyf vtmfddysat lpllivvile
      541 niavsfvygi dkfledltdm lgfapskyyy ymwkyisplm lvtlliasiv nmglsppgyn
      601 awikekasee flsypmwgmv vcfslmvlai lpvpvvfvir rcnliddssg nlasvtykrg
      661 rvlkepvnld gddaslihgk ipsemsspnf gkniyrkqsg sptldtapng rygigylmad
      721 mpdmpesdl
//
1..729
/gene="Slc6a15"
/gene_synonym="Ntt73"
Feature Q08469 : 1 segment
Display: FASTA GenBank Help
Details

Supplemental Content

Change region shown

Customize view

Reference sequence information

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
External link. Please review our privacy policy.