LOCUS NM_001199637 6446 bp mRNA linear PRI 03-FEB-2019
DEFINITION Homo sapiens ADCYAP receptor type I (ADCYAP1R1), transcript variant
4, mRNA.
ACCESSION NM_001199637
VERSION NM_001199637.1
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6446)
AUTHORS Shintani Y, Hayata-Takano A, Moriguchi K, Nakazawa T, Ago Y, Kasai
A, Seiriki K, Shintani N and Hashimoto H.
TITLE beta-Arrestin1 and 2 differentially regulate PACAP-induced PAC1
receptor signaling and trafficking
JOURNAL PLoS ONE 13 (5), e0196946 (2018)
PUBMED 29734363
REMARK GeneRIF: These results show that b-arrestin1 and b-arrestin2 exert
differential actions on PAC1R internalization and PAC1R-dependent
ERK1/2 activation, and suggest that the two b-arrestin isoforms may
be involved in fine and precise tuning of the PAC1R signaling
pathways.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 6446)
AUTHORS Bonaventura G, Iemmolo R, D'Amico AG, La Cognata V, Costanzo E,
Zappia M, D'Agata V, Conforti FL, Aronica E and Cavallaro S.
TITLE PACAP and PAC1R are differentially expressed in motor cortex of
amyotrophic lateral sclerosis patients and support survival of
iPSC-derived motor neurons
JOURNAL J. Cell. Physiol. 233 (4), 3343-3351 (2018)
PUBMED 28926110
REMARK GeneRIF: that perturbations in the PACAP-PAC1R pathway may be
involved in amyotrophic lateral sclerosis pathology
REFERENCE 3 (bases 1 to 6446)
AUTHORS King SB, Toufexis DJ and Hammack SE.
TITLE Pituitary adenylate cyclase activating polypeptide (PACAP), stress,
and sex hormones
JOURNAL Stress 20 (5), 465-475 (2017)
PUBMED 28610473
REMARK GeneRIF: Studies suggest that the level of stress and circulating
gonadal hormones may differentially regulate the PACAPergic system
in males and females to influence anxiety-like behavior and may be
one mechanism underlying the discrepancies in human psychiatric
disorders. [Review Article]
Review article
REFERENCE 4 (bases 1 to 6446)
AUTHORS Lind MJ, Marraccini ME, Sheerin CM, Bountress K, Bacanu SA,
Amstadter AB and Nugent NR.
TITLE Association of Posttraumatic Stress Disorder With rs2267735 in the
ADCYAP1R1 Gene: A Meta-Analysis
JOURNAL J Trauma Stress 30 (4), 389-398 (2017)
PUBMED 28746747
REMARK GeneRIF: These results provide evidence for an association between
ADCYAP1R1 and PTSD and indicate that there may indeed be sex
differences.
Review article
REFERENCE 5 (bases 1 to 6446)
AUTHORS Liao C, Zhao X, Brewer M, May V and Li J.
TITLE Conformational Transitions of the Pituitary Adenylate
Cyclase-Activating Polypeptide Receptor, a Human Class B GPCR
JOURNAL Sci Rep 7 (1), 5427 (2017)
PUBMED 28710390
REMARK GeneRIF: Transitions between different conformational states of the
G protein-coupled pituitary adenylate cyclase-activating
polypeptide receptor (PAC1R) range between microseconds to
milliseconds.
Publication Status: Online-Only
REFERENCE 6 (bases 1 to 6446)
AUTHORS Pantaloni C, Brabet P, Bilanges B, Dumuis A, Houssami S, Spengler
D, Bockaert J and Journot L.
TITLE Alternative splicing in the N-terminal extracellular domain of the
pituitary adenylate cyclase-activating polypeptide (PACAP) receptor
modulates receptor selectivity and relative potencies of PACAP-27
and PACAP-38 in phospholipase C activation
JOURNAL J. Biol. Chem. 271 (36), 22146-22151 (1996)
PUBMED 8703026
REFERENCE 7 (bases 1 to 6446)
AUTHORS Pisegna JR and Wank SA.
TITLE Cloning and characterization of the signal transduction of four
splice variants of the human pituitary adenylate cyclase activating
polypeptide receptor. Evidence for dual coupling to adenylate
cyclase and phospholipase C
JOURNAL J. Biol. Chem. 271 (29), 17267-17274 (1996)
PUBMED 8663363
REFERENCE 8 (bases 1 to 6446)
AUTHORS Stoffel M, Espinosa R 3rd, Trabb JB, Le Beau MM and Bell GI.
TITLE Human type I pituitary adenylate cyclase activating polypeptide
receptor (ADCYAP1R): localization to chromosome band 7p14 and
integration into the cytogenetic, physical, and genetic map of
chromosome 7
JOURNAL Genomics 23 (3), 697-699 (1994)
PUBMED 7851900
REFERENCE 9 (bases 1 to 6446)
AUTHORS Ohtaki T, Masuda Y, Ishibashi Y, Kitada C, Arimura A and Fujino M.
TITLE Purification and characterization of the receptor for pituitary
adenylate cyclase-activating polypeptide
JOURNAL J. Biol. Chem. 268 (35), 26650-26657 (1993)
PUBMED 8253796
REFERENCE 10 (bases 1 to 6446)
AUTHORS Ogi K, Miyamoto Y, Masuda Y, Habata Y, Hosoya M, Ohtaki T, Masuo Y,
Onda H and Fujino M.
TITLE Molecular cloning and functional expression of a cDNA encoding a
human pituitary adenylate cyclase activating polypeptide receptor
JOURNAL Biochem. Biophys. Res. Commun. 196 (3), 1511-1521 (1993)
PUBMED 7902709
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from DA809763.1, AK226138.1,
AC006466.3 and CA432770.1.
[WARNING] On Jun 2, 2019 this sequence was replaced by
NM_001199637.2.
Summary: This gene encodes type I adenylate cyclase activating
polypeptide receptor, which is a membrane-associated protein and
shares significant homology with members of the glucagon/secretin
receptor family. This receptor mediates diverse biological actions
of adenylate cyclase activating polypeptide 1 and is positively
coupled to adenylate cyclase. Multiple alternatively spliced
transcript variants encoding distinct isoforms have been
identified. [provided by RefSeq, Dec 2010].
Transcript Variant: This variant (4) lacks two consecutive exons in
the upstream region and one exon in the downstream region in the
CDS, as compared to variant 1. The resulting isoform (3) lacks two
internal segments, as compared to isoform 1.
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data to make the sequence consistent with the
reference genome assembly. The genomic coordinates used for the
transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803616.144268.1, AK226138.1
[ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1968968, SAMEA2142680
[ECO:0000348]
##Evidence-Data-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-414 DA809763.1 1-414
415-1955 AK226138.1 349-1889
1956-6250 AC006466.3 66528-70822
6251-6446 CA432770.1 1-196 c
FEATURES Location/Qualifiers
source 1..6446
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="7"
/map="7p14.3"
gene 1..6446
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/note="ADCYAP receptor type I"
/db_xref="GeneID:117"
/db_xref="HGNC:HGNC:242"
/db_xref="MIM:102981"
exon 1..218
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
misc_feature 212..214
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/note="upstream in-frame stop codon"
exon 219..340
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
CDS 290..1633
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/note="isoform 4 precursor is encoded by transcript
variant 4; pituitary adenylate cyclase activating
polypeptide 1 receptor type I Hiphop; PACAP-R1; PACAP type
I receptor; PACAP receptor 1; adenylate cyclase activating
polypeptide 1 (pituitary) receptor type I; pituitary
adenylate cyclase-activating polypeptide type 1 receptor"
/codon_start=1
/product="pituitary adenylate cyclase-activating
polypeptide type I receptor isoform 4 precursor"
/protein_id="NP_001186566.1"
/db_xref="CCDS:CCDS56481.1"
/db_xref="GeneID:117"
/db_xref="HGNC:HGNC:242"
/db_xref="MIM:102981"
/translation="MAGVVHVSLAALLLLPMAPAMHSDCIFKKEQAMCLEKIQRANEL
MGFNDSSPGCPGMWDNITCWKPAHVGEMVLVSCPELFRIFNPDQDMGVVSRNCTEDGW
SEPFPHYFDACGFDEYESETGDQDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKL
HCTRNFIHMNLFVSFMLRAISVFIKDWILYAEQDSNHCFISTVECKAVMVFFHYCVVS
NYFWLFIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVCVTVWATLRLYFDDTGCW
DMNDSTALWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYLRLARST
LLLIPLFGIHYTVFAFSPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQAEIKR
KWRSWKVNRYFAVDFKHRHPSLASSGVNGGTQLSILSKSSSQIRMSGLPADNLAT"
sig_peptide 290..358
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="COORDINATES: ab initio prediction:SignalP:4.0"
exon 341..446
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 447..554
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 555..664
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 665..762
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 763..895
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 896..1049
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1050..1110
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1111..1180
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1181..1272
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1273..1402
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1403..1444
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
exon 1445..6428
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
/inference="alignment:Splign:2.1.0"
regulatory 6403..6408
/regulatory_class="polyA_signal_sequence"
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
polyA_site 6428
/gene="ADCYAP1R1"
/gene_synonym="PAC1; PAC1R; PACAPR; PACAPRI"
ORIGIN
1 gcgagcgtgg tccccgcgtg cgcacacgca cacgccgccg cgcagggaca cacggacccc
61 ggccgccagc cggccacaca ggcgcggaga cccggctcgg cgcgcgctcc tcggcgcaca
121 cgctccccat ccccgcgccg cgcgggccgc ggacttgcag gctgcgcgtc ctttgcggag
181 tctgccccgg ccccacggac aggccccgca gtgagcagcc cagagacaca ttggggctga
241 cctgccgctg ctgtcagtgg gaggccagtg gtgctggcca agaagtgtca tggctggtgt
301 cgtgcacgtt tccctggctg ctctcctcct gctgcctatg gcccctgcca tgcattctga
361 ctgcatcttc aagaaggagc aagccatgtg cctggagaag atccagaggg ccaatgagct
421 gatgggcttc aatgattcct ctccaggctg tcctgggatg tgggacaaca tcacgtgttg
481 gaagcccgcc catgtgggtg agatggtcct ggtcagctgc cctgagctct tccgaatctt
541 caacccagac caagacatgg gagtggtgag ccggaactgc acggaggatg gctggtcgga
601 acccttccct cattactttg atgcctgtgg gtttgatgaa tatgaatctg agactgggga
661 ccaggattat tactacctgt cagtgaaggc cctctacacg gttggctaca gcacatccct
721 cgtcaccctc accactgcca tggtcatcct ttgtcgcttc cggaagctgc actgcacacg
781 caacttcatc cacatgaacc tgtttgtgtc gttcatgctg agggcgatct ccgtcttcat
841 caaagactgg attctgtatg cggagcagga cagcaaccac tgcttcatct ccactgtgga
901 atgtaaggcc gtcatggttt tcttccacta ctgtgttgtg tccaactact tctggctgtt
961 catcgagggc ctgtacctct tcactctgct ggtggagacc ttcttccctg aaaggagata
1021 cttctactgg tacaccatca ttggctgggg gaccccaact gtgtgtgtga cagtgtgggc
1081 tacgctgaga ctctactttg atgacacagg ctgctgggat atgaatgaca gcacagctct
1141 gtggtgggtg atcaaaggcc ctgtggttgg ctctatcatg gttaactttg tgctttttat
1201 tggcattatc gtcatccttg tgcagaaact tcagtctcca gacatgggag gcaatgagtc
1261 cagcatctac ttgcgactgg cccggtccac cctgctgctc atcccactat tcggaatcca
1321 ctacacagta tttgccttct ccccagagaa tgtcagcaaa agggaaagac tcgtgtttga
1381 gctggggctg ggctccttcc agggctttgt ggtggctgtt ctctactgtt ttctgaatgg
1441 tgaggtacaa gcggagatca agcgaaaatg gcgaagctgg aaggtgaacc gttacttcgc
1501 tgtggacttc aagcaccgac acccgtctct ggccagcagt ggggtgaatg ggggcaccca
1561 gctctccatc ctgagcaaga gcagctccca aatccgcatg tctggcctcc ctgctgacaa
1621 tctggccacc tgagccatgc tcccctcctc ctcctctcct ccatccacag gctgggaccg
1681 caggcaggtg ccagcccacg catgtttgcg cctcttccct ccccttgggc aggccctggg
1741 ctggaagctt ggctcctgag gggggagaag gaggcagggc acagactgga attgtcccct
1801 ccttgttttg gtactggtcc cacccactgt ggtcccctgg gccctgaccc cagacatgta
1861 aatactcctc aaatttggaa aagttgtccc atcccttccc cctttgcccc agtgtccctg
1921 ctcacttcca gtcaggtctc agctccaccc cactgtttct tggtcagcct cagtgatggc
1981 ctgaccccag ctgtgaggcc ttgtgagcta aggctgaaga gaccttgcct gtgggagctg
2041 gggtggtacc tgccccaaca gccccccatc atctgatttt gggtgtgacc cctcgagtgt
2101 gcatgccacc ctggggctct tctcagtcct gggcccaagt ccctgaagcc tagcagaggg
2161 ccagctcggg tggccagaac accccttggt gcaggcctcc tggcactcgc ctggcatctt
2221 ggtgtccggg tgctcagctc ctgggtccca gggcagtggg acagctccca ggctcttctc
2281 atcagagact tcagtttggg tggggagtct gcaggtcagg gaaggttctg gtgcttttcg
2341 tttgatccaa aaggagctga gagccagaaa tgtggctgtg attgcgaaag agacggagtt
2401 tgggagaccc atggaagaaa accttcgcca tctccattgt tttaccccca gctcaagatg
2461 gacagggttc ttctggtcat ctcccttcca cctcttccac cctcatggat gccttccaga
2521 agctgtctcc aaccatgcac accacagaga gcccttaagc cctcccctcc ccatttcccc
2581 taagtcaaag ccatgactag gatgtaccag ccacctgcct ctgatgtcgg cgaatcctga
2641 agcacctgca cccgaatctg gggcccaggg ctgcccgacc cccactccca tggatgatga
2701 ggagccccag acaccagctc ctctggcctg gactcaacta ttgaaatctc tctgagacct
2761 ctgcaaagat gtccctttta tgcccttgac cttgactcac catgggctgc tggggtgtcg
2821 ggagaagcca cacccaacat gcccattagc agaggctgct gctgatgtgg cagctgctcc
2881 ctaccacccg ccctggctcc ccagctgcag gttgcacacc ctcagcctga gaatgtaaaa
2941 tagggcagtg cccagctggg gaggtagagg aaagtgctag agtacatggt ccgtgtgtgg
3001 cctagctcat tccatgtcag catttggtgt tactcctgtc caaattctgt ttgcagactt
3061 gtttcatgat gatgctctgg aatgcttggg gactttgggt ggaggaggat gttacttcat
3121 tttataaaga ggaaggggcc tgggagactt gggaaaatgt ttctggagag gctttcagag
3181 tggctggaaa aggctcttgt tgctgtaatt tagagaggag ctctgtgtcc ttggccagtc
3241 aagtaagttc ctggatcttt ggcaaagcca gaggaagaaa ggagctacct gccctttcct
3301 gggcatgctt ggtgactcag attgatcttg gagtgtgcag tggcccttct accctgcacc
3361 tgacctcatg atcccctcca cctgcactgg tgtgcattac tgaagcaagg gtcctgtgga
3421 catcttgcat tttgggcact gctagggggt ctgggctgct tggaaggagc catggcctag
3481 ggttcaggga actagactct atcccagttg ggttcagact gctgtgactg tggacacgat
3541 gctccttgtt ctgggctcca gttttcccat ttgtgagatg aggcaacttc caatgcttct
3601 ctaggccctg cacgtaagtg atttttccag ttcaaagtca agacaggtaa aagggcattc
3661 atcattgtgc ctcatatcag gttttgatga atatatgtgt gtgtgtgtgt gtgtgtgtgt
3721 gtgtgtgtgt agagggatgg acaggatgct gtttattttc cctttcttgg aaatggacct
3781 tctgtccctt ccatttggac accacagtgg aagctggtgg cctggaagga aggattaggt
3841 catggacatt tgaacaggtg ccttgggcat gatgtataga tgcagtcata tataccttgc
3901 tggggtgggg tgccacctcc agtggccagc tccagatcca aggagcagcc ccctggggat
3961 ggaccccatt cattcatcat gactcccaac agttttcatt gtggaagaag aaactttggc
4021 attatagaga catcatcaca aaacagtaaa aacaaaatca accctgaacc ccatgcactg
4081 tgctgcttcc tgagcctggc cctgcatcct catagaggtg ccgggttcct attggttagt
4141 tggttgtttt tccgtctgag tgaatttttg ccagtcttgt gagcagatgt acctgatgta
4201 ttctcaatgt tccaagaggt tctggccttc agggtcacag gcagtagggg gacagcataa
4261 ggtctatgta aaacccttcc ctctctgacc ctctgttttc aaatctgtaa aatgggcaat
4321 aagactagat gatttgtata tagcccaatg catctctgga actctgtcta aacaccagcc
4381 atctacttgg aatgggcccc aggactgtgg tatttgcctg ggccaggaaa ggataagaaa
4441 tcctgtcatg tgaagacagc ttgagaggct tgagaaaagt ggggctgggg agaagcaggc
4501 ttgtcagact ccacccctgt tgatgatcat tcctgggaag gggtttctcg ttctatgcaa
4561 tcctaaagga cgaaactcac ccatgggagg ccgaattctc cttgggatga agaaatttct
4621 ctttccctgt catgagtgtc cagccaggga gcagggaggc agtgtcaggg agggactctc
4681 atcctggagg aaatgggatt ccaagtcaag gatgctgagg ctgtcaggga gccagagagg
4741 ggggtccaag tgcgggatgt gggtggctct gtggttcagt ggctctgtgg tagttcctag
4801 cactgcagac ttcatgactc cccacttaag tccaagtcac attgtctatc ccagtgtgta
4861 gctctgtcac cctgcttgac acatccagtg gcctacagcg actcttctct aaccccaccc
4921 cctccaagct gggttctttg tggaagaagg acagggagct agagccaagc cctaggcttg
4981 agagacacct gcatctataa tccccgccaa ggatgcccac tcacctctct catctgatcc
5041 tcactctttg tggaagggaa agctcaaagg gactctctct ctctctctct tttttttttt
5101 ttttgagtag tacccttgcc ctcttcatgg ccacttcaaa gtgaagccag caaagtgata
5161 atactttatc atttagtatt atcataaagt attaatactt tgtcataaag tcctccttga
5221 gcccagggac catggaagtc agctagaaga gccctgagca aggagcaagg acttgggctt
5281 ctccacgctt tgctcctggc ttgtttgacc ttgactcatt ccccatatgt ctttgaggag
5341 gctcacaaaa tactaaagct gggaggaaac ttggagatct ataggtcaaa cctccccatt
5401 gggctgatga gaaaatacac gcaggcctag catggtgcct gccaccatgg tgggatccag
5461 tatgttttat aaatctgaat gagtaaatgg ctcaccaatt tatgcatagc cctgcacatg
5521 agcagaatgt gacactcaaa gcatccatgc agtacgcatg taaccttgca caggagtggg
5581 gctctggtga ccgaaggttg tccaggactc ttgcaggaga agcaatggag tcagtgtggt
5641 gtggggagac ctacttttta acctgggctt agccacctgc tctgtgatcc agggcttacc
5701 ttctttgggc ctcggcctcc taatctgggt aatggggagg acttcattgg cattgttagt
5761 cccacaggcc aaggataagg ttgaaatgag acggcttgtg tgtgaaaaga ttttggaaat
5821 tacacagatg tgggcttgtt attgggatga agactgctgg aagggactcc ttgctgttta
5881 tctactgctt tgagccctcc taagttaacc tgtgcctcat ttgtaaaacc accagcatca
5941 ggagtaaggg ggaggccaga gggctcagat ggacacagaa ttctagcttt acctgcatcc
6001 gctgattcag ttttctgttg ggatcagagt gaggatactt ccatatgggt gatagcagcc
6061 atgcccctgg gagtcaactt caaggatctg ggacattttg gtgtgcccat tccttctttt
6121 cctgaactca cagtcttggg gtgtttctgc acttggctat gtgtgtcttg tctgatgtct
6181 gtcttctgta gctttgcctc tatcagggct ggagtggtgc agcccctggc atctcagaca
6241 tggttcctgc ctcacttgtg ggagctggac cagcctgggt ttcatctccc acagtaaagc
6301 taagtaagcc ccacagacct tactgctact gctgctgcca ttaatgctgt gctcactatc
6361 ttgtccagga ttttaaggat gtcagactgc tgtagatgac tcaataaatg ttttgccatt
6421 tttcctggaa aaaaaaaaaa aaaaaa
//