|
| Status |
Public on Sep 05, 2012 |
| Title |
Cheng_576-5_7-days_light_Rep1_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
7d light-grown Arabidopsis Col-0 seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: seedling
treatment: light
|
| Treatment protocol |
The control was grown in the dark; the hydrogen peroxide sample was grown in the dark and treated with 5mM H2O2; the light sample was grown in the light.
|
| Growth protocol |
Arabidopsis Col-0 seeds were grown on half MS media for 7 d, and then the seedlings were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen RNeasy Plant Mini Kit under the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from 7d light-grown Col-0 seedlings under 16 h light / 8 h dark photoperiod.
|
| Data processing |
The chip data were normalized by the RMA method and analyzed using the affy and limma packages in R/Bioconductor.
|
| |
|
| Submission date |
Sep 04, 2012 |
| Last update date |
Sep 05, 2012 |
| Contact name |
Han Cheng |
| Organization name |
The Chinese University of Hong Kong
|
| Department |
School of Life Science
|
| Street address |
Shatin
|
| City |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE40574 |
Expression data from Arabidopsis under hydrogen peroxide and light treatment |
|
