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Sample GSM98270

Query DataSets for GSM98270

Status

Public on Feb 23, 2007

Title

40%sugar_LS_2

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in unsugared dough.

Extracted molecule

polyA RNA

Extraction protocol

Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 40% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

[email protected]

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	68.26	51.91	3,543	P
1769309_at	0.146	15.22	2.216	A
1769310_at	0.403	15.22	6.137	A
1769311_at	0.727	1,104	803	P
1769312_at	1.128	134.5	151.7	P
1769313_at	5.412	87.04	471	P
1769314_at	0.957	2,178	2,084	P
1769315_at	0.0263	15.22	0.4	A
1769316_s_at	0.0259	15.22	0.394	A
1769317_at	0.596	528.2	315	P
1769318_at	0.646	15.22	9.838	A
1769319_at	1.36	421.3	573	P
1769320_at	2.026	195.6	396.2	P
1769321_at	0.323	281.4	90.89	P
1769322_s_at	0.258	210.6	54.27	P
1769323_at	0.961	413.6	397.2	P
1769324_at	1.04	234.1	243.5	P
1769325_at	2.145	27.35	58.66	P
1769326_at	0.402	15.22	6.116	A
1769327_at	0.0513	15.22	0.781	A

Total number of rows: 10928

Table truncated, full table size 331 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98270.CEL.gz	1.5 Mb	(ftp)(http)	CEL
GSM98270.CHP.gz	57.3 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file