|
| Status |
Public on Nov 06, 2013 |
| Title |
rph1-YPD-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
W303 background, rph1, YPD, log phase
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain/background: W303
genotype: rph1 deletion
medium: rich
growth phase: log
|
| Treatment protocol |
The cell pellets were harvested by centrifugation and washed twice with TBS pH 7.4. The pellets were stored at -80C.
|
| Growth protocol |
Yeasts were grown in rich medium (YPD) at 30C to log phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by the Geneaid MiniRNA kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16.5 hr at 45C on Yeast Genome 2.0. GeneChips were washed and stained in the Affymetrix® GeneChip® Fluidics Station 450 according to the Mini_euk2v3_450 protocol.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip® Scanner 3000.
|
| Description |
BH239-2
Gene expression profile from rph1-deleted strain.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The further analysis was performed using GeneSpring 11.
|
| |
|
| Submission date |
Aug 07, 2012 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Wan-Sheng Lo |
| E-mail(s) |
[email protected]
|
| Phone |
(02)27871039
|
| Organization name |
Academia Sinica
|
| Department |
IPMB
|
| Lab |
A422
|
| Street address |
128 Academia Road, Section 2, Nankang
|
| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE39950 |
Expression data from wild-type and rph1-deleted yeasts |
|
