|
| Status |
Public on Oct 01, 2012 |
| Title |
Heart.Placebo.IR.3 |
| Sample type |
RNA |
| |
|
| Source name |
ventricular myocardium, 5 days placebo, ischemia-reperfused
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: male
age: 10-14 weeks
agent: placebo
treatment protocol (post-agent): global normothermic ischemia and aerobic reperfusion
tissue: ventricular myocardium
|
| Treatment protocol |
Mature male C57Bl/6 mice were briefly anesthetized with halothane, a small incision made at the base of the neck, and placebo or 75-mg morphine pellets were inserted into the dorsal subcutaneous space before closure with 9-mm wound clips. Pellets were left in place for 5 days before analysis of cardiac I-R tolerance ex vivo. Briefly mice were anesthetized with sodium pentobarbital and hearts excised and perfused in a Langendorff mode. After 30 min stabilization control (placebo) and SLP hearts were subjected to either 25 min of global normothermic ischemia and 45 minutes of aerobic reperfusion, or time-matched normoxic perfusion. Hearts were collected in RNAlater and the left ventricular tissue dissected from the heart.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit from Ambion
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 15
replicate 3
|
| Data processing |
The data were variance stabilized and robust spline normalized using the ‘lumi’ package in R/BioConductor
|
| |
|
| Submission date |
Jul 17, 2012 |
| Last update date |
Oct 01, 2012 |
| Contact name |
Kevin John Ashton |
| E-mail(s) |
[email protected]
|
| Phone |
+61-7-55954198
|
| Organization name |
Bond University
|
| Department |
Health Sciences & Medicine
|
| Street address |
University Drive
|
| City |
Gold Coast |
| State/province |
QLD |
| ZIP/Postal code |
4229 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE39407 |
Transcriptional profile of sustained ligand-activated preconditioning in pre- and post-ischemic myocardium |
|
