|
| Status |
Public on Feb 14, 2006 |
| Title |
SOCS1-/-IFNg-/- + 12h IFNg |
| Sample type |
RNA |
| |
|
| Source name |
SOCS1-/-IFNg-/- liver + 12h IFNg
|
| Organism |
Mus musculus |
| Characteristics |
Suppressor of Cytokine Signalling 1-/-, Interferon-gamma-/- liver
|
| Treatment protocol |
Mice were IP injected with 100 ul (4ug) of recombinant interferon-gamma or 100 ul of phosphate-buffered saline (PBS), and livers were taken at 0h, 0.5h, 1h, 2h, 4h, 12h, 16h, 24h or 48h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen), and cleaned with RNeasy (Qiagen)
|
| Label |
biotin-phycoerythrin
|
| Label protocol |
Biotin-labelled cRNA was generated as instructed by Affymetrix
|
| |
|
| Hybridization protocol |
Samples were hybridized to MGU74Av2 microarrays as instructed by Affymetrix
|
| Scan protocol |
Samples were scanned using an Affymetrix GeneArray Scanner, as instructed by Affymetrix
|
| Description |
-
|
| Data processing |
Raw probe data were quantile-normalized, background corrected and summarized using the Affy package for the R statistical programming environment
|
| |
|
| Submission date |
Feb 12, 2006 |
| Last update date |
Feb 13, 2006 |
| Contact name |
Sam Wormald |
| E-mail(s) |
[email protected]
|
| Phone |
03-9345-2525
|
| Organization name |
The Walter and Eliza Hall Institute
|
| Department |
Cancer and Haematology
|
| Lab |
Hilton Lab
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3050 |
| Country |
Australia |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE4232 |
Interferon-gamma timecourse in IFNg-/- and SOCS1-/-IFNg-/- mouse livers |
|
