Mice were IP injected with 100 ul (4ug) of recombinant interferon-gamma or 100 ul of phosphate-buffered saline (PBS), and livers were taken at 0h, 0.5h, 1h, 2h, 4h, 12h, 16h, 24h or 48h.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with Trizol (Invitrogen), and cleaned with RNeasy (Qiagen)
Label
biotin-phycoerythrin
Label protocol
Biotin-labelled cRNA was generated as instructed by Affymetrix
Hybridization protocol
Samples were hybridized to MGU74Av2 microarrays as instructed by Affymetrix
Scan protocol
Samples were scanned using an Affymetrix GeneArray Scanner, as instructed by Affymetrix
Description
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Data processing
Raw probe data were quantile-normalized, background corrected and summarized using the Affy package for the R statistical programming environment
