|
| Status |
Public on Mar 01, 2006 |
| Title |
Adp300mut T0 |
| Sample type |
RNA |
| |
|
| Source name |
C2-derived myoblast
|
| Organism |
Mus musculus |
| Characteristics |
C3H strain
C2 myoblasts stably expressing antisense IGF-II cDNA (C2AS12 cells)
|
| Growth protocol |
Cells were infected with recombinant adenoviruses encoding either wild type or mutant p300 in the presence or absence doxycline and were incubated in low serum differentiation medium for 24 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent with additional Na_acetate/EtOH precipitation
|
| Label |
biotin
|
| Label protocol |
~8 ug total RNA was labeled using standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Adp300mut infected C2AS12 cells prior to addition of DM
|
| Data processing |
Chip data (.cel files) were normalized using global scaling values (200), and analyzed with Affymetrix Microarray Suite 5.0 software using default parameters except for a Tau setting of 0.015. Filtered pair-wise comparisons were performed with the following criteria: only expression signal values greater or equal to 100 for Ad-p300 wild type samples were considered and mean fold-change greater or equal to 4 vs both Ad-p300 +Dox and Ad-p300deltaTAZ2 samples was required for selection.
|
| |
|
| Submission date |
Feb 09, 2006 |
| Last update date |
Feb 10, 2006 |
| Contact name |
David Kuninger |
| Phone |
503 494 0539
|
| Fax |
503 494 8393
|
| Organization name |
Oregon Health & Science University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Rotwein
|
| Street address |
3181 SW Sam Jackson Park Rd
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239-3098 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE4224 |
p300-mediated gene expression during muscle cell survival |
|
