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| Status |
Public on Jul 28, 2006 |
| Title |
ACC 3 cell line lacZ-RNAi replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
ACC3 cell line, Sox4 RNAi knockdown with lacZ as control
|
| Organism |
Homo sapiens |
| Characteristics |
Biological Material: Cell line, grown invitro; Tissue derivation: Adenoid cystic carinoma of the parotid gland
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Affymetrix: Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Affymetrix: Expression Analysis Technical Manual
|
| Scan protocol |
Affymetrix: Expression Analysis Technical Manual
|
| Description |
shRNA sequences present in pENTR plasmid vector transfected by electroporation (0.35 kV, with 550 _F capacitance). RNA harvested 48 hours after electroportation.
|
| Data processing |
dCHIP 2006 (Li and Wong)
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| |
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| Submission date |
Feb 09, 2006 |
| Last update date |
Jul 28, 2006 |
| Contact name |
Christopher A. Moskaluk |
| E-mail(s) |
[email protected]
|
| Phone |
434 982-4408
|
| Fax |
434 243-6757
|
| Organization name |
Christopher Moskaluk
|
| Department |
Pathology
|
| Street address |
P.O. Box 800214, OMS Rm. 3890, Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4225 |
Sox4 knockdown in ACC3 cells |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
dCHIP (2006) Calculated expression value |
| VALUE_SE |
dCHIP (2006) Calculated standard error (SE) of expression value |
| ABS_CALL |
dCHIP (2006) present (P), absent (A), marginal (M) |
| Data table |
| ID_REF |
VALUE |
VALUE_SE |
ABS_CALL |
| AFFX-BioB-5_at |
91.8167 |
4.71725 |
P |
| AFFX-BioB-M_at |
93.1037 |
9.10002 |
P |
| AFFX-BioB-3_at |
93.8302 |
4.74146 |
P |
| AFFX-BioC-5_at |
150.085 |
9.18541 |
P |
| AFFX-BioC-3_at |
244.525 |
7.78268 |
P |
| AFFX-BioDn-5_at |
271.453 |
16.5647 |
P |
| AFFX-BioDn-3_at |
1107.93 |
53.9646 |
P |
| AFFX-CreX-5_at |
3047.93 |
174.398 |
P |
| AFFX-CreX-3_at |
3292.6 |
107.417 |
P |
| AFFX-DapX-5_at |
5.92095 |
1.07464 |
A |
| AFFX-DapX-M_at |
8.8413 |
1.98166 |
A |
| AFFX-DapX-3_at |
5.24505 |
1.01495 |
A |
| AFFX-LysX-5_at |
4.71647 |
0.929202 |
A |
| AFFX-LysX-M_at |
11.728 |
1.17662 |
A |
| AFFX-LysX-3_at |
14.2268 |
0.988049 |
A |
| AFFX-PheX-5_at |
6.14693 |
0.79353 |
A |
| AFFX-PheX-M_at |
2.53343 |
0.815497 |
A |
| AFFX-PheX-3_at |
19.7212 |
1.67182 |
A |
| AFFX-ThrX-5_at |
18.7562 |
1.14945 |
A |
| AFFX-ThrX-M_at |
5.17147 |
0.696882 |
A |
Total number of rows: 22283
Table truncated, full table size 625 Kbytes.
| Supplementary data files not provided |
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