|
| Status |
Public on May 03, 2013 |
| Title |
N2_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Caenorhabditis elegans wild type
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: Bristol N2
|
| Treatment protocol |
Untreated
|
| Growth protocol |
Mixed stage C. elegans strains were cultured at 20◦C on solid NGM agar plates on HT115(DE3) E. coli as a food source for 3-4 days. For each replicate worms from 10-20 9-cm NGM plates were collected using sucrose floatation. Starved plates were discarded.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nematodes were disrupted using Mini-Beadbeater (BioSpec Products) in TRIZOL (Invitrogen) and total RNA was extracted according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
biotin using Genechip 3' IVT Express kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization protocol according to manufacturers instruction (Affymetrix)
|
| Scan protocol |
Scan protocol according to manufacturers instruction (Affymetrix)
|
| Description |
Untreated mixed stage N2 strain
|
| Data processing |
Pre-processing using the software Expression Console (Affymetrix). Processing algorithm: Global Median, following Background correction (PM-GCBG) and the summarization method of Plier.
|
| |
|
| Submission date |
Jul 11, 2012 |
| Last update date |
May 03, 2013 |
| Contact name |
Jessica Margareta Lindvall |
| E-mail(s) |
[email protected]
|
| Organization name |
SciLifeLab. Stockholm University
|
| Department |
NBIS
|
| Street address |
Tomtebodavägen 23A
|
| City |
Solna |
| ZIP/Postal code |
11111 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE39252 |
Expression changes in Caenorhabditis elegans xpa-1 mutant |
|
