|
| Status |
Public on Jul 07, 2012 |
| Title |
NMIBC tissue of patient No.7 |
| Sample type |
RNA |
| |
|
| Source name |
NMIBC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 55
bc tumor stage: Ta
ethnic group: Chinese Han
tissue: bladder
sample type: NMIBC
patient no: 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
A total of 500-1000ng of total RNA was labelled using the Genisphere HSR labelling Kit (http://www.genisphere.com/array_detection_flashtag_biotin.html).
|
| |
|
| Hybridization protocol |
The total amount of RNA was hybridized to the arrays for 16 hours at 48C with rotation at 60rpm.
|
| Scan protocol |
Samples were scanned using the GCS 3000 7G Scanner and processed using miRNA_1-0_2xgain library files.
|
| Description |
Gene expression data from NMIBC tissue
|
| Data processing |
The data was preprocessed using the Affymetrix miRNA QC tool where Individual feature intensities were flagged by detection calls, background subtracted, transformed through addition of a small constant and median summarized.
|
| |
|
| Submission date |
Jul 03, 2012 |
| Last update date |
Jul 07, 2012 |
| Contact name |
qinsong zeng |
| E-mail(s) |
[email protected]
|
| Organization name |
Changhai Hospital
|
| Street address |
168 Changhai Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE39093 |
Expression data from bladder tumors and adjacent normal tissues |
|
