|
| Status |
Public on Jun 16, 2012 |
| Title |
4wk Heart 4 |
| Sample type |
RNA |
| |
|
| Source name |
4 wks_Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 4 weeks
growth rate: slowly proliferating
tissue: Heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
4w8H
|
| Data processing |
RMA nomanization method of Partek Genomics Suite Version 6.6
|
| |
|
| Submission date |
Jun 15, 2012 |
| Last update date |
Jun 16, 2012 |
| Contact name |
Julian CK Lui |
| E-mail(s) |
[email protected]
|
| Phone |
301-496-8049
|
| Organization name |
NICHD, NIH
|
| Lab |
Section on Growth and Development
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38754 |
Temporal changes of gene expression in mouse heart, kidney and lung during juvenile growth |
|
