|
| Status |
Public on Jun 15, 2016 |
| Title |
Jurkat_TRAIL_resistant_clone3_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
TRAIL resistant
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Jurkat-TR3
genotype/variation: TRAIL resistant
cell type: T cell leukemia / lymphoma
|
| Treatment protocol |
none
|
| Growth protocol |
Cells were cultured in Iscove's modified Dulbecco's medium (IMDM) supplemented with 10% (FBS) and 1% penicillin/streptomycin. TRAIL-resistant Jurkat clones (TR1, TR2, TR3) were established by selective pressure of TRAIL 1000 ng/mL over the period of 12 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated from biologic duplicates of untreated Jurkat cells (WT) and TRAIL resistant Jurkat cell line subclones (TR1, TR2, TR3) using RNAblue (Top-Bio, Czech Republic) according to the manufaccturer’s instructions. RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technology). Extracted RNA was amplified using the Illumina RNA Amplification Kit (Ambion) and hybridized to the Illumina HumanRef-8 BeadChip (Illumina).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
Data analysis was performed in R-software, in beadarray and limma packages from BioConductor project.
|
| |
|
| Submission date |
Jun 15, 2012 |
| Last update date |
Jun 15, 2016 |
| Contact name |
Robert Ivanek |
| Phone |
+41 61 207 35 41
|
| Organization name |
University of Basel
|
| Department |
Department of Biomedicine
|
| Lab |
Bioinformatics
|
| Street address |
Hebelstrasse 20
|
| City |
Basel |
| ZIP/Postal code |
4053 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE38743 |
Acquired resistance of Jurkat cells to TNF-related apoptosis-inducing ligand (TRAIL) |
|
