|
| Status |
Public on Jun 14, 2012 |
| Title |
human dark zone, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
human tonsil dark zone cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue source: pediatric tonsil specimens
cell type: Germinal center B cells (CD19+CD38+IgD-)
cell subpopulation: dark zone (CXCR4lo/CD83hi)
|
| Growth protocol |
Germinal center cells were isolated from pediatric tonsil specimens
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133plus2 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned on Affymetrix GeneChip Scanner 3000 7G using Command Console Software.
|
| Description |
HUMAN_DZ1
|
| Data processing |
The data were RMA-normalized using Gene Pattern Expression File Creator Software (v.7.0).
|
| |
|
| Submission date |
Jun 13, 2012 |
| Last update date |
Jun 14, 2012 |
| Contact name |
Gabriel D Victora |
| E-mail(s) |
[email protected]
|
| Organization name |
Whitehead Institute
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE38697 |
Gene expression in human germinal center light zone and dark zone B cells |
| GSE38712 |
Gene expression in germinal center light zone and dark zone B cells |
|
