|
| Status |
Public on Aug 21, 2012 |
| Title |
ASXL1siRNA3 |
| Sample type |
RNA |
| |
|
| Source name |
Normal CD34+ Cord Blood
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: ASXL1siRNA
|
| Treatment protocol |
CD34+ cells were transfected with shRNA for ASXL1 or vector control after 24 hours in culture using the 4D-Nucleofector system according to the manufacturer's protocol from Lonza (Walkersville, MD)
|
| Growth protocol |
CD34+ cells were cultured in serum-free stem cell expansion media (StemSPAN SFEM; StemCell Technologies) supplemented with SCF (100 ng/mL), IL-3 (20 ng/mL), and IL-6 (10 ng/mL), every other day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIZOL according to manufacturers guidelines
|
| Label |
biotin
|
| Label protocol |
Please refer to Affymetrix 3' IVT Express Kit Documentaion (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
|
| |
|
| Hybridization protocol |
Please see AffymetrixExpression Analysis Technical Manual (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
|
| Scan protocol |
Please see AffymetrixExpression Analysis Technical Manual (http://www.affymetrix.com/estore/browse/products.jsp?categoryIdClicked=&productId=131415#1_3)
|
| Description |
ASXL1 short hairpin
|
| Data processing |
RMA : log2 transform : Quantile Normalization : Median Polish using Partek Genomics Suite Version 6.5
|
| |
|
| Submission date |
Jun 13, 2012 |
| Last update date |
Aug 21, 2012 |
| Contact name |
Omar Abdel-Wahab |
| E-mail(s) |
[email protected]
|
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Department |
HOPP
|
| Lab |
Dr. Ross Levine
|
| Street address |
1275 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE38692 |
ASXL1 Knock Down in Normal CD34+ Cord Blood and UKE1 Cell Lines |
|
