|
| Status |
Public on Jan 29, 2006 |
| Title |
2408day7_20Gy |
| Sample type |
RNA |
| |
|
| Source name |
THP-1 cells irradiated
|
| Organism |
Homo sapiens |
| Characteristics |
myelomonocytic leukemia cell line
|
| Biomaterial provider |
LGC Promochem, Wesel, Germany
|
| Treatment protocol |
irradiated with 20Gy
|
| Growth protocol |
cultivated in RPMI1640 medium, 10% FCS, 1mM L-glutamine, 1% penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini-Kit including Qiashredder columns (Qiagen)
|
| Label |
biotin labeled
|
| Label protocol |
one cycle target labeling assay
|
| |
|
| Hybridization protocol |
according to affymetrix
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000
|
| Description |
THP-1 cells harvested 7 days post irradiation
|
| Data processing |
GCOS 1.4.0.036 scaled to 500
|
| |
|
| Submission date |
Jan 27, 2006 |
| Last update date |
Jan 27, 2006 |
| Contact name |
Henning Witt |
| E-mail(s) |
[email protected]
|
| Phone |
++49-30-450578727
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Hans Lehrach
|
| Lab |
Patricia Ruiz
|
| Street address |
Ihnestrasse 65
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4110 |
Effect of ionizing radiation on procoagulant activity |
|
