|
| Status |
Public on Mar 31, 2006 |
| Title |
Ept2 DES-treated replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
anterior pituitary
|
| Organism |
Rattus norvegicus |
| Characteristics |
Ept2 congenic rat, male, age 21 weeks, treated with the synthetic estrogen diethylstilbestrol (DES) for 12 weeks
|
| Treatment protocol |
diethylstilbestrol (DES) treatment for 12 weeks starting at 9 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Anterior pituitary gland was harvested at the time of sacrifice, weighed and flash-frozen in liquid nitrogen. Tissue was homogenized in lysis buffer by passage through an 18- and then 22-guage needle. Total RNA was isolated using the Absolutely RNA Kit from Stratagene.
|
| Label |
biotin
|
| Label protocol |
Approximately 2 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Ept2 congenic rats were generated by introgressing COP alleles onto an ACI background within the Ept2 locus on rat chromosome 3. At 9 weeks of age, a silastic tubing implant containing 5mg of the synthetic estrogen diethylstilbestrol (DES) was surgically implanted subcutaneously in the interscapular region of male rats. Each animal received an intraperitoneal injection of BrdU (50mg/kg) 4 hours prior to sacrifice. Animals were sacrificed at 21 weeks of age by decapitation and pituitary glands were immediately harvested, weighed and flash-frozen in liquid nitrogen. Tissues were homogenized through a 22-gauge needle and total RNA was extracted using the Absolutely RNA Kit (Stratagene). A total of 3 DES-treated males were used, resulting in two independent biological replicates.
|
| Data processing |
Affymetrix GeneChip Operating Software version 1.2
|
| |
|
| Submission date |
Jan 23, 2006 |
| Last update date |
Nov 27, 2012 |
| Contact name |
James Shull |
| E-mail(s) |
[email protected]
|
| Phone |
608-265-5003
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
McArdle Laboratory for Cancer Research
|
| Lab |
Shull Laboratory
|
| Street address |
1400 University Avenue
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE4080 |
Effect of DES-treated Ept congenic rat lines on gene expression in the anterior pituitary gland. |
| GSE4083 |
Diethylstilbestrol effects on rat anterior pituitary gland |
|
