|
| Status |
Public on May 21, 2012 |
| Title |
4hr WT rep1 |
| Sample type |
RNA |
| |
|
| Source name |
4hr WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: female
tissue: lung
|
| Treatment protocol |
Seven-week-old female C57BL/6J mouse (Jackson Laboratories, Wilmington, MA) was inoculated via the intranasal route with Staphylococcus aureus Newman wild-type (WT).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mouse lung RNA was isolated by directly solubilizing lung tissue in Trizol LS (Life Technologies) using a Brinkman Polytron tissue disruptor.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized with Affymetrix Mouse Genome 430 2.0 Arrays per manufacturer’s instructions.
|
| Scan protocol |
Hybridizations were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from lung tissue of mouse infected with WT S. aureus at 4-hour time point.
|
| Data processing |
The expression level was summarized by gcrma package in Bioconductor with GC robust multichip average (GCRMA) normalization.
|
| |
|
| Submission date |
May 18, 2012 |
| Last update date |
May 21, 2012 |
| Contact name |
Tong Zhou |
| Organization name |
University of Nevada School of Medicine
|
| Street address |
1664 N Virginia St
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38053 |
Host Response Signature to Staphylococcus aureus alpha-Hemolysin |
|
