NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM92584 Query DataSets for GSM92584
Status Public on Apr 01, 2006
Title Mouse sarcoma replicate 2
Sample type RNA
 
Channel 1
Source name mouse sarcoma
Organism Mus musculus
Characteristics mouse sarcoma that developed spontaneously on an intact mouse. This was used as a control for contamination of the human xenografts with mouse stromal cells.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description mouse sarcoma that developed spontaneously on an intact mouse. This was used as a control for contamination of the human xenografts with mouse stromal cells.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 1.14 6728.708995 3024.462151 3680.662857 2059.162264
2 -0.36 3319.58 2942.166667 2358.36 2007.226667
3 -1.33 4043.682353 2942.2 4084.142222 2040.093023
4 0.33 4079.266187 2962.199288 2624.427481 2014.304498
5 2875.100917 2937.010309 1934.752294 1966.593103
6 2914.93578 2924.872247 1956.899083 1973.638767
7 2951.669725 2933.992308 1981.577982 1982.624535
8 2919.302752 2928.418118 1962.917431 1969.058419
9 2916.887097 2933.70347 1975.419355 1977.709779
10 2929.986207 2926.527415 1973.875862 1982.762402
11 2948.302326 2934.892157 1988.127907 1969.27451
12 2964.12844 2939.552408 1989.46789 1970.215297
13 2944.055172 2953.192727 1977.144828 1967.745455
14 2930.440367 2943.426295 1986.592593 1966.438247
15 2967.321101 2948.253968 1972.66055 1977.151927
16 2967.577982 2968.122066 1954.348624 2003.273585
17 2935.302752 2965.564246 1972.587156 1995.944134
18 2939.967742 2919.669516 1989.096774 1981.988604
19 2937.387097 2952.851351 1979.387097 1988.945946
20 2956.807339 2959.025126 1977.06422 1992.582915

Total number of rows: 19200

Table truncated, full table size 1039 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap