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Sample GSM92583 Query DataSets for GSM92583
Status Public on Apr 01, 2006
Title Mouse sarcoma replicate 1
Sample type RNA
 
Channel 1
Source name mouse sarcoma
Organism Mus musculus
Characteristics mouse sarcoma that developed spontaneously on an intact mouse. This was used as a control for contamination of the human xenografts with mouse stromal cells.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description mouse sarcoma that developed spontaneously on an intact mouse. This was used as a control for contamination of the human xenografts with mouse stromal cells.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 24, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 1.04 4709.21466 2487.934718 3738.796209 1831.375394
2 -0.09 2687.628866 2439.112329 2207.063492 1813.613095
3 -1.12 3390.373134 2447.052288 5221.990909 1886.136364
4 0.44 3233.746269 2445.357724 2712.946746 1841.099526
5 2431.435484 2411.5 1789.919355 1789.710938
6 2435.112903 2417.662722 1786.274194 1784.473373
7 2430.22093 2419.665722 1780.72093 1782.113314
8 2423.613793 2411.158774 1788.993103 1791.961003
9 2426.112903 2412.899408 1781.112903 1787.656805
10 2425.678756 2410.213904 1784.093264 1783.967914
11 2437.284404 2413.74502 1799.853211 1788.541833
12 2419.06422 2432.871795 1786.880734 1804.37037
13 2431.883721 2423.420074 1783.383721 1793.553903
14 2435.779817 2423.508353 1793.614679 1792.582339
15 2453.403226 2427.52071 1795.170732 1795.60355
16 2431.409326 2428.459893 1805.865285 1802.417112
17 2458.752294 2423.661631 1797.522936 1791.009063
18 2436.116279 2424.771704 1792.837209 1804.419672
19 2454.220183 2423.024055 1789.229358 1785.690722
20 2424.12844 2432.151203 1799.449541 1791.841924

Total number of rows: 19200

Table truncated, full table size 1042 Kbytes.




Supplementary data files not provided

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