NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM92578 Query DataSets for GSM92578
Status Public on Apr 01, 2006
Title PC xenograft PC324 after castration replicate_2
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, without expression of a functional androgen receptor, 10 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.12 3473.85 1844.085714 6027.673469 3313.549488
2 -0.2 2173.490446 1718.789625 3961.912281 3085.016575
3 0.39 4669.352273 1715.973684 6250.337209 3183.214286
4 -0.12 2572.130435 1707.165563 4754.931034 3115.098765
5 1780.036697 1783.939241 3115.688073 2957.898734
6 1768.041451 1772.960352 3073.067358 2930.555066
7 1706.018519 1741.149321 3010.277778 2989.782805
8 1647.33945 1654.508711 3078.018349 2981.254355
9 1617.889908 1633.58481 3049.321101 3011.260759
10 1596.788991 1565.649485 3065.633028 2962.247423
11 1594.259067 1584.597015 3043.098446 3040.238806
12 1605.144828 1599.877814 3118.689655 3044.958199
13 1611.284404 1607.02439 3099.798165 2942.859107
14 1588.53886 1589.791822 2955.637306 2962.594796
15 1626.418605 1586.262195 3058.581395 2969.530488
16 1616.954128 1555.885196 2953.394495 2964.912387
17 1572.110092 1577.92068 3027.119266 2974.492918
18 1626.112903 1581.7151 3155.435484 2972.250712
19 1537.258065 1532.842893 2960.16129 2919.371571
20 1511.853211 1508.060423 3086.93578 2968.012085

Total number of rows: 19185

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap