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Sample GSM92572 Query DataSets for GSM92572
Status Public on Apr 01, 2006
Title PC xenograft PC135 after castration replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, without expression of a functional androgen receptor, 14 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.39 3624.955414 1635.827957 5981.030769 3199.684211
2 1783.592233 1589.748466 3171.633028 3014.169184
3 -0.94 2132.915789 1620.321951 4092.083333 3051.458824
4 -0.06 2397 1630.559406 3941.685039 3082.987212
5 1589.77931 1581.981395 3131.296552 2983.841121
6 1574.091743 1577.804878 3184.825688 3049.679443
7 1577.076923 1604.85283 3127.504587 3034.089888
8 1722.330275 1665.345725 3188.642202 3166.64684
9 1814.20202 1780.565916 3452.844037 3221.903537
10 1850.934426 1799.015789 3510.612903 3323.753927
11 2011.46789 1808.653386 3551.541284 3309.171315
12 1874.912621 1788.832714 3497.651376 3221.234201
13 1882.29703 1735.263456 3455.798165 3237.501416
14 1881.197917 1866.882591 3460.725389 3411.894737
15 2040.53886 1926.663968 3805.772021 3664.728745
16 1936.256881 1876.675241 3540.055046 3457.569132
17 1753.376147 1696.315186 3183.651376 3204.141643
18 1602.403226 1624.34375 3036.629032 3078.46875
19 1624.055046 1615.309973 3126.055046 2960.172507
20 1628.269767 1622.486891 2996.12037 3006.719101

Total number of rows: 19185

Table truncated, full table size 1039 Kbytes.




Supplementary data files not provided
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