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Sample GSM92556 Query DataSets for GSM92556
Status Public on Apr 01, 2006
Title PC xenograft PC346I after castration replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, androgen receptor expressing, 15 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.02 4013.168224 1633.585903 6478.887701 4139.952756
2 -0.35 2101.803922 1575.315556 4635.889908 4032.550186
3 0.82 5261.223529 1665.271565 6214.203125 4256.96338
4 -0.39 2572.150538 1580.572327 5253.323308 3975.204545
5 1545.668394 1542.02139 4006.528497 3975.315508
6 1538.110092 1539.3829 3901.229358 3869.412639
7 1532.662791 1518.202381 4061.848837 3781.535714
8 1534.495413 1522.241901 4092.825688 3828.506494
9 1536.642202 1529.798489 4074.166667 3877.697733
10 1532.348624 1520.995671 4105.412844 3944.372294
11 1525.009174 1511.521036 3803.357798 3989.436893
12 1513.367442 1519.048689 4037.694444 3839.235955
13 1535.669725 1532.749004 4020.275229 3893.051793
14 1523.367876 1518.855019 3974.632124 3951.197026
15 1527.046632 1530.291498 4087.865285 3949.838057
16 1548.568807 1528.306569 4072.12844 3896.676399
17 1519.614679 1542.252788 3884.183486 3987.598513
18 1544.962963 1543.477352 3846.238532 4000.543554
19 1532.951724 1542.120172 3958.333333 3861.304721
20 1523.854839 1525.884984 3975.048387 3864.996805

Total number of rows: 19185

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
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