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Sample GSM92552 Query DataSets for GSM92552
Status Public on Apr 01, 2006
Title PC xenograft PC346B after castration replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, androgen receptor expressing, 13 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.32 4208.392405 1692.921986 6732.830986 2490.38255
2 0.24 2694.476923 1679.937143 3650.018182 2383.940541
3 0.27 5483.070064 1726.883392 6602.478873 2435.691275
4 -0.09 2703.530303 1664.489247 3936.348624 2333.331646
5 1647.614679 1642.404313 2332.296296 2270.409704
6 1652.677419 1626.858108 2284.854839 2246.304054
7 1649.774194 1647.509749 2301.129032 2282.301676
8 1636.513761 1643.395498 2269.834862 2236.160772
9 1659.798165 1671.338645 2246.385321 2243.713147
10 1672.912281 1665.048951 2264.034884 2236.475862
11 1654.612903 1643.142145 2282.822581 2228.508728
12 1660.073394 1641.072961 2221.853211 2354.538117
13 1678.822581 1674.254545 2225.274194 2256.530909
14 1662.791667 1670.898785 2256.984456 2192.275304
15 1697.305556 1700.463415 2218.888889 2198.390244
16 1688.53211 1709.490085 2248.220183 2275.88102
17 1675.972414 1662.627306 2291.544828 2271.592727
18 1625.613793 1642.851735 2277.006897 2271.116719
19 1616.631579 1632.112045 2247.197674 2258.218487
20 1614.293578 1628.821002 2217.724771 2288.200477

Total number of rows: 19185

Table truncated, full table size 1042 Kbytes.




Supplementary data files not provided
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