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Sample GSM92550 Query DataSets for GSM92550
Status Public on Apr 01, 2006
Title PC xenograft PC346 after castration replicate_2
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-responsive xenograft, androgen receptor expressing, 6 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.44 6279.678832 3656.719472 7819.043956 2766.364341
2 -0.02 4877.260504 3678.215743 4406.601307 2703.909091
3 0.51 9561.271739 3756.796875 8412.829268 2782.161702
4 -0.22 4679.903846 3704.312849 4275.296875 2699.51497
5 3759.798165 3692.183607 2712.605505 2666.013115
6 3771.192661 3695.807365 2708.752294 2645.161473
7 3791.006897 3724.195804 2691.393103 2660.391608
8 3745.266055 3731.141994 2714.752294 2643.311178
9 3671.968912 3660.317181 2640.860104 2654.334802
10 3664.740741 3677.00813 2695.12037 2677.073171
11 3759.247706 3708.855305 2730.40367 2676.617363
12 3713.541284 3736.334661 2672.201835 2698.247012
13 3710.401606 3722.170543 2650.072289 2654.775194
14 3714.104651 3676.848276 2655.383721 2653.351724
15 3694.939759 3711.287958 2692.040323 2671.13089
16 3667.064516 3676.036364 2682.580645 2691.905455
17 3632.605505 3678.985836 2650.752294 2695.354108
18 3650.709677 3631.624691 2688.903226 2728.439506
19 3631.62963 3636.471074 2633.284404 2735.266932
20 3741.170984 3677.358491 2729.067358 2710.503145

Total number of rows: 19185

Table truncated, full table size 1043 Kbytes.




Supplementary data files not provided
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