NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM92548 Query DataSets for GSM92548
Status Public on Apr 01, 2006
Title PC xenograft PC346 after castration replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-responsive xenograft, androgen receptor expressing, 10 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.44 6554.592593 3876.598684 8009.944828 2808.081356
2 0.04 4890.695652 3851.864615 4143.373134 2749.013072
3 0.61 10905.23256 3987.164179 9168.497354 2863.386454
4 -0.05 4941.238095 3910.25788 4250.380952 2769.261972
5 3855.577982 3835.942721 2646.256881 2679.704057
6 3805.596774 3779.648649 2673.516129 2683.304054
7 3432.220183 3667.24406 2624.238532 2662.388769
8 3768.387097 3651.540541 2700.129032 2647.701695
9 3747.951613 3704.706806 2688.467742 2655.570681
10 3665.449541 3705.960159 2658.385321 2694.828685
11 3702.60241 3606.773333 2708.763052 2654.853333
12 3685 3586.14511 2700.306452 2692.769716
13 3619.944954 3590.609836 2690.073394 2652.754098
14 3669.541284 3583.95122 2688.66055 2684.808362
15 3626.612903 3580.779944 2687.822581 2681.89415
16 3642.642202 3594.845921 2691.284404 2720.223565
17 3630.906977 3600.308682 2704.546512 2667.356913
18 3644.386207 3583.92429 2738.951724 2688.277603
19 3615.365517 3613.742373 2663.351724 2688.745763
20 3630.337349 3577.483444 2676.313253 2682.993377

Total number of rows: 19185

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap