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Sample GSM92542 Query DataSets for GSM92542
Status Public on Apr 01, 2006
Title PC xenograft PC310 after castration replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-dependent xenograft, androgen receptor expressing, 12 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) [email protected]
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.11 5127.396825 1716.289855 9636.04878 2206.019608
2 0.3 2121.28 1698.456522 3152.591549 2067.275
3 0.98 6610.811594 1701.455782 7198.72093 2160.283186
4 0.56 2723.206349 1586.987342 4325.972603 2107.53719
5 1558.945946 1568.884615 2131.844156 2006.828283
6 1574.683544 1554.168675 2087.679012 2050.833333
7 1537.655914 1514.152381 1967.247525 2045.978261
8 1522.179487 1515.014085 2118.219178 1974.740741
9 1548.765625 1545.283019 2032.636364 2051.098039
10 1525.313253 1539.083333 2027.878788 2164.32
11 1527.314286 1522.022222 2106.985915 2034.206897
12 1544.111888 1531.4 2051.138889 2130.05
13 1523.225806 1514.12 2046.106383 2009.655172
14 1515.298507 1515.674419 2040.322581 2092.102564
15 1523.024793 1547.105263 2043.283333 2122.105263
16 1509.546667 1522.90625 2058.345679 2021.874016
17 1526.744186 1525.927711 2056.263158 2086.073171
18 1530.169492 1536.241379 2041.008547 2059.2
19 1538.518519 1529.863636 2060.816901 1986.395062
20 1516.243902 1515.268817 2059.348837 2071.340206

Total number of rows: 19185

Table truncated, full table size 1021 Kbytes.




Supplementary data files not provided
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