|
| Status |
Public on Apr 26, 2012 |
| Title |
MCAD 5 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: mcad -/-
gender: Male
genetic background: BL6/SV129 mixed
age: 50-60 days of age
diet: 12 hrs fasted
treatment: vehicle
tissue: liver
|
| Treatment protocol |
Wild type and mcad-/- mice where injected with vehicle or lps and subsequently fasted for 12 hrs , liver was isolated and RNA was extracted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRI -reagent was used to isolate RNA, and Qiagen Rneasy columns where used to purify the RNA
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocols where used
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols where used
|
| Scan protocol |
Standard Affymetrix protocols where used
|
| Data processing |
The data where normalised using gcRMA normalisation protocols using MADMAX analysis pipeline pmid: 21778530
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
Apr 26, 2012 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE37546 |
Disturbed Hepatic Carbohydrate Management During High Metabolic Demand in Medium-Chain Acyl-CoA Dehydrogenase (MCAD)-deficient Mice |
|
