|
| Status |
Public on Jan 05, 2008 |
| Title |
Leaf_sorbitol1 |
| Sample type |
RNA |
| |
|
| Source name |
leaf
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
control for sucrose feeding (leaf_sucrose1)
ecotype: Col-0
developmental stage: full grown leaves of non-flowering plants
|
| Treatment protocol |
Source leaves were cut at the end of the day from non-flowering plants. Leaf peteoles were recut under buffer (2mM MES, pH6.5) and placed in buffer + 100mM sorbitol for 13hours in the dark. After incubation discs were snap-frozen in liquid nitrogen.
|
| Growth protocol |
Plants were grown in a phytotron with a 14h day / 10h night. Temperature: 20°C / 16°C. Humidity: 60% / 75%. Lightintensity: .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using TRIzol Total RNA Isolation Reagent, according to the protocol supplied by the manufacturer GibcoBRL Life-Technologies.
|
| Label |
biotin
|
| Label protocol |
10µg total RNA was biotin labelled
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
source leaves of Wild type Arabidopsis thaliana Col0 rosette plants.
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Jan 12, 2006 |
| Last update date |
Jan 08, 2007 |
| Contact name |
Joost T. van Dongen |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0)331 5678353
|
| URL |
http://www.mpimp-golm.mpg.de
|
| Organization name |
Max Planck Institute of Molecular Plant Physiology
|
| Street address |
Am Muehlenberg 1
|
| City |
Potsdam |
| ZIP/Postal code |
D-14476 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE4022 |
effect of sucrose feeding to detached leaves |
|
