|
| Status |
Public on Oct 15, 2014 |
| Title |
CTRL_sponge-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T-lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
construct: Control
|
| Treatment protocol |
CD4+ naïve T-lymphocytes were stimulated by anti CD3-CD28 beads(dynal) at a 1:1 cell to beads ratio. 5 days later (48 hrs before harvest) cells were re-stimulated with the same protocol
|
| Growth protocol |
primary T-lymphocytes were grown in RPMI supplemented with 10% FBS, L-glutamine, pen/strep and IL-2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Tri-reagent (Sigma) according o manufacturer instructions
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression kit was used according to manufacturer instructions
|
| |
|
| Hybridization protocol |
GeneChip WT labeling and Hybridization kit from Ambion was used according to manufacturer instructions
|
| Scan protocol |
GeneChips were scanned using the 7G Scanner
|
| Description |
N/A
|
| Data processing |
Affymetrix .CEL files were separately processed for each replicate using the Robust Multi-array Average (RMA) algorithm with quantile normalization as implemented in the Bioconductor package 'oligo'.
|
| |
|
| Submission date |
Apr 12, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Teresa Colombo |
| Organization name |
Consiglio Nazionale delle Ricerche (CNR)
|
| Department |
Institute of Molecular Biology and Pathology (IBPM)
|
| Street address |
p.le A. Moro 7
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE37213 |
MiR-21 function in T-lymphocytes |
|
| Relations |
| Reanalyzed by |
GSE86357 |
