|
| Status |
Public on Mar 30, 2012 |
| Title |
Standard diet 3 |
| Sample type |
RNA |
| |
|
| Source name |
standard chow (lean group)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: mesenteric adipose tissue surrounding the pancreas (pMES)
diet: Standard diet
|
| Treatment protocol |
Animals were divided into two dietary sets for 30 days. One group was fed with standard chow diet (supplying 8% of calories as fat; type AO4 from Panlab, Barcelona, Spain). The second group was fed with a cafeteria diet (66% of calories as fat), as previously described (Endocrinology 146:4362–4369, 2005).
|
| Growth protocol |
Adult male Wistar rats (Charles River Laboratories, Wilmington, MA), 7 wk old (weighing 225–250 g), were caged individually in a 12-h light, 12-h dark cycle in a temperature- and humidity-controlled environment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy minikit (QIAGEN, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized, washed and stained according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
|
| Description |
Gene expression data from the mesenteric adipose tissue surrounding the pancreas (pMES) in standard diet fed Wistar rats for 30 days
|
| Data processing |
Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package from Bioconductor on R language.
|
| |
|
| Submission date |
Mar 29, 2012 |
| Last update date |
Feb 18, 2013 |
| Contact name |
Jordi Altirriba |
| E-mail(s) |
[email protected]
|
| Organization name |
Centre Médical Universitaire de Genève
|
| Department |
Dpts de Médecine Interne et de Physiologie cellulaire et Métabolisme
|
| Lab |
Laboratoire du Métabolisme
|
| Street address |
Rue Michel-Servet 1
|
| City |
Genève |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE36935 |
Role of IGFBP-3 in the Regulation of β-Cell Mass during Obesity: Adipose Tissue/ β-Cell Cross Talk |
|
| Relations |
| Reanalyzed by |
GSM1084015 |
