|
| Status |
Public on Jan 01, 2006 |
| Title |
Drosophila_T0-4 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila embryos before nuclear cycle 9 (maternal transcripts)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Genotype: yellow white and Oregon R parents Age : embryos younger than nuclear cycle 9, i.e. before pole cells budding
|
| Treatment protocol |
Embryos were dechorionated with 50% bleach, put on a cover slip and covered with Halocarbon oil 27 (Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
30 min egg collections of OreR and yw flies at 25°C were aged at room temperature (RT) according to the different temporal classes T0-T4.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer’s instructions.
|
| Label |
biotine
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45oC on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation. File T0-4.
|
| Data processing |
The image data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 30, 2005 |
| Last update date |
Feb 23, 2007 |
| Contact name |
Fanny PILOT |
| E-mail(s) |
[email protected]
|
| Phone |
33 491 26 96 20
|
| Fax |
33 491 82 06 82
|
| Organization name |
UMR6545 CNRS-Université de la Méditerrannée
|
| Department |
Institut de Biologie du Développement de Marseille (IBDM)
|
| Lab |
Laboratoire de Génétique et de Physiologie du Développement
|
| Street address |
Campus de Luminy case 907
|
| City |
Marseille cedex 9 |
| ZIP/Postal code |
13288 |
| Country |
France |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3955 |
Expression data from early Drosophila embryo |
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