|
| Status |
Public on Feb 05, 2013 |
| Title |
Benign prostate tissue 20 |
| Sample type |
RNA |
| |
|
| Source name |
Bening prostate tissue 20
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Benign, prostate tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Total RNA (100ng per sample) was labeled using the FlashTag Biotin HSR kit (Genisphere)
|
| |
|
| Hybridization protocol |
According to manufacturer's protocol.
|
| Scan protocol |
Probe level (.CEL file) data were generated using Affymetrix Command Console v1.1
|
| Description |
benign prostate tissue
|
| Data processing |
Arrays were processed using the GCOS software. Data were extracted from the Affymatrix miRNA QC tool using RMA background correction and median polish summarization. Variance Stabilization Normalization (VSN) procedure was carried out for global noralization
|
| |
|
| Submission date |
Mar 26, 2012 |
| Last update date |
Feb 06, 2013 |
| Contact name |
Pei-Chun Lin |
| E-mail(s) |
[email protected]
|
| Phone |
(212)746-6306
|
| Fax |
(212)746-8816
|
| Organization name |
Cornell Weill Medical College
|
| Department |
Pathology and Laboratory Medicine
|
| Lab |
Dr. Mark A. Rubin
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE36802 |
microRNA profiling in paired human prostate cancer and benign prostate tissue |
| GSE36803 |
Paired human prostate cancer and benign prostate tissues, and LNCaP cells treated with miR-31 |
|
