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Status
Public on Apr 08, 2006
Title
Proloferation at 4 hours (B)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens Characteristics
HMVECs induced with VEGF
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto gelatin–coated plates and the cells were allowed to attach for 4 hours. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed by 1:
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with VEGF
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
[email protected] Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL97 Series (1)
GSE3891 Endothelial Cell Morphogenesis
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
362
A
0.0727
AFFX-BioB-M_at
355.9
P
0.013811
AFFX-BioB-3_at
216.7
A
0.239063
AFFX-BioC-5_at
1014.8
P
0.000581
AFFX-BioC-3_at
720.1
P
0.004017
AFFX-BioDn-5_at
878
P
0.000581
AFFX-BioDn-3_at
6193.6
P
0.001248
AFFX-CreX-5_at
7598.9
P
0.000044
AFFX-CreX-3_at
14531.3
P
0.000044
AFFX-DapX-5_at
90.4
M
0.062929
AFFX-DapX-M_at
18.7
A
0.544587
AFFX-DapX-3_at
12.8
A
0.969024
AFFX-LysX-5_at
31.8
A
0.631562
AFFX-LysX-M_at
33.5
A
0.686277
AFFX-LysX-3_at
14.9
A
0.544587
AFFX-PheX-5_at
11.2
A
0.916408
AFFX-PheX-M_at
24.4
A
0.794268
AFFX-PheX-3_at
53.8
A
0.772364
AFFX-ThrX-5_at
16
A
0.963431
AFFX-ThrX-M_at
107.6
A
0.51489
22645 592 Kbytes .Supplementary data files not provided
