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Status
Public on Apr 08, 2006
Title
Tubulogenesis at 2 hours (B)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens
Characteristics
HMVECs induced with matrigel
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto Matrigel™ – coated plates and the cells were allowed to attach for 2 hours. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed b
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with matrigel
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
[email protected]
Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL97
Series (1)
GSE3891
Endothelial Cell Morphogenesis
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
568.7
M
0.058444
AFFX-BioB-M_at
783.7
P
0.003595
AFFX-BioB-3_at
470.8
A
0.102165
AFFX-BioC-5_at
1653.5
P
0.000581
AFFX-BioC-3_at
951.6
P
0.001102
AFFX-BioDn-5_at
1417.9
P
0.00011
AFFX-BioDn-3_at
8623.5
P
0.000581
AFFX-CreX-5_at
12991.6
P
0.000044
AFFX-CreX-3_at
25952.9
P
0.000044
AFFX-DapX-5_at
170.7
P
0.033677
AFFX-DapX-M_at
56.6
A
0.617401
AFFX-DapX-3_at
76.4
A
0.737173
AFFX-LysX-5_at
61.1
A
0.51489
AFFX-LysX-M_at
259.8
A
0.455413
AFFX-LysX-3_at
21.9
A
0.672921
AFFX-PheX-5_at
16.5
A
0.772364
AFFX-PheX-M_at
36.9
A
0.760937
AFFX-PheX-3_at
231.9
A
0.354453
AFFX-ThrX-5_at
46.6
A
0.876428
AFFX-ThrX-M_at
131.6
A
0.440646
Total number of rows: 22645 Table truncated, full table size 592 Kbytes .Supplementary data files not provided