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Status
Public on Apr 08, 2006
Title
Proloferation at 4 hours (A)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens Characteristics
HMVECs induced with VEGF
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto gelatin–coated plates and the cells were allowed to attach for 4 hours. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed by 1:1 phenol/chloroform and ethanol precipitation. RNA integrity was monitored using an Agilent 2100 Bioanalyzer.
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with VEGF
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
[email protected] Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL96 Series (1)
GSE3891 Endothelial Cell Morphogenesis
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
201.8
A
0.067678
AFFX-BioB-M_at
161.5
M
0.058444
AFFX-BioB-3_at
55
A
0.41138
AFFX-BioC-5_at
415
P
0.000662
AFFX-BioC-3_at
282.4
P
0.003212
AFFX-BioDn-5_at
342.7
P
0.001102
AFFX-BioDn-3_at
2496.8
P
0.000754
AFFX-CreX-5_at
2933.2
P
0.000044
AFFX-CreX-3_at
4826.2
P
0.000044
AFFX-DapX-5_at
41.9
A
0.116113
AFFX-DapX-M_at
23.1
A
0.544587
AFFX-DapX-3_at
7.6
A
0.945787
AFFX-LysX-5_at
30.9
A
0.48511
AFFX-LysX-M_at
19.5
A
0.559354
AFFX-LysX-3_at
11.8
A
0.354453
AFFX-PheX-5_at
3.9
A
0.981719
AFFX-PheX-M_at
5.7
A
0.9273
AFFX-PheX-3_at
24.1
A
0.712257
AFFX-ThrX-5_at
9.7
A
0.843268
AFFX-ThrX-M_at
10.9
A
0.783476
22283 593 Kbytes .Supplementary data files not provided
