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Status
Public on Apr 08, 2006
Title
Proloferation at 30 minutes (A)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens Characteristics
HMVECs induced with VEGF
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto gelatin–coated plates and the cells were allowed to attach for 30 minutes. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed by 1:1 phenol/chloroform and ethanol precipitation. RNA integrity was monitored using an Agilent 2100 Bioanalyzer.
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with VEGF
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
[email protected] Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL96 Series (1)
GSE3891 Endothelial Cell Morphogenesis
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
106.9
A
0.095667
AFFX-BioB-M_at
135.7
P
0.010317
AFFX-BioB-3_at
98.3
A
0.131361
AFFX-BioC-5_at
411.8
P
0.000754
AFFX-BioC-3_at
267.1
P
0.004017
AFFX-BioDn-5_at
410.6
P
0.000857
AFFX-BioDn-3_at
2345.8
P
0.000662
AFFX-CreX-5_at
2738.8
P
0.000044
AFFX-CreX-3_at
5253.8
P
0.000052
AFFX-DapX-5_at
29.4
A
0.440646
AFFX-DapX-M_at
10
A
0.699394
AFFX-DapX-3_at
8.7
A
0.794268
AFFX-LysX-5_at
14.6
A
0.631562
AFFX-LysX-M_at
79.1
A
0.250796
AFFX-LysX-3_at
30.7
A
0.440646
AFFX-PheX-5_at
46.7
A
0.631562
AFFX-PheX-M_at
7.6
A
0.916408
AFFX-PheX-3_at
16.9
A
0.814869
AFFX-ThrX-5_at
9.4
A
0.672921
AFFX-ThrX-M_at
48
A
0.5
22283 593 Kbytes .Supplementary data files not provided
