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Status
Public on Apr 08, 2006
Title
Tubulogenesis at 4 hours (A)
Sample type
RNA
Source name
HMVEC
Organism
Homo sapiens Characteristics
HMVECs induced with matrigel
Extracted molecule
total RNA
Extraction protocol
1.35E6 HMVEC cells were plated onto Matrigel™ – coated plates and the cells were allowed to attach for 4 hours. Cells were collected by scraping, washed with PBS, and RNA prepared using Trizol (Invitrogen) extraction followed by 1:1 phenol/chloroform and ethanol precipitation. RNA integrity was monitored using an Agilent 2100 Bioanalyzer.
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
HMVEC induced with matrigel
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 21, 2005
Last update date
Mar 10, 2006
Contact name
suneeta mandava
E-mail(s)
[email protected] Organization name
Argonne National Lab
Department
Bio
Lab
Rodi
Street address
9700 S Cass ave
City
Argonne
State/province
IL
ZIP/Postal code
60439
Country
USA
Platform ID
GPL96 Series (1)
GSE3891 Endothelial Cell Morphogenesis
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
232.5
P
0.018281
AFFX-BioB-M_at
204
P
0.002556
AFFX-BioB-3_at
122.3
M
0.062929
AFFX-BioC-5_at
506.1
P
0.00034
AFFX-BioC-3_at
372.4
P
0.002867
AFFX-BioDn-5_at
570.2
P
0.000195
AFFX-BioDn-3_at
3034.5
P
0.000509
AFFX-CreX-5_at
4138.8
P
0.000044
AFFX-CreX-3_at
7602.9
P
0.000044
AFFX-DapX-5_at
39.3
A
0.147939
AFFX-DapX-M_at
30.8
A
0.368438
AFFX-DapX-3_at
4.9
A
0.960339
AFFX-LysX-5_at
1.8
A
0.814869
AFFX-LysX-M_at
35.7
A
0.470241
AFFX-LysX-3_at
11.6
A
0.327079
AFFX-PheX-5_at
4.5
A
0.910522
AFFX-PheX-M_at
4.6
A
0.973889
AFFX-PheX-3_at
12.5
A
0.794268
AFFX-ThrX-5_at
9.1
A
0.783476
AFFX-ThrX-M_at
24.5
A
0.645547
22283 592 Kbytes .Supplementary data files not provided
