|
| Status |
Public on Mar 07, 2012 |
| Title |
Keratinocytes_TNF_subject1 |
| Sample type |
RNA |
| |
|
| Source name |
primary keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary keratinocytes
treatment: TNF-treated
|
| Treatment protocol |
Cultures were starved of growth factors in unsupplemented M154 medium (Invitrogen/Cascade Biologics, Portland, OR) for 24 hours before use. Cultures were stimulated 24 hours with recombinant human cytokines and growth factors from R&D Systems (Minneapolis, MN).
|
| Growth protocol |
Monolayer NHK cultures were established and used in the second or third passage (Elder 1991, J. Invest. Dermatol. 96: 425-433). Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNEasy mini kits
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
|
| Description |
primary human keratinocytes treated with TNF
|
| Data processing |
Robust Multichip Average (RMA)
|
| |
|
| Submission date |
Mar 05, 2012 |
| Last update date |
Mar 07, 2012 |
| Contact name |
William R Swindell |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard University
|
| Department |
Genetics
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE36287 |
Expression data from primary human keratinocytes exposed to cytokines in vitro (IL-4, IL-13, IL-17A, IFN-alpha, IFN-gamma, TNF) |
|
