Primary fibroblasts were obtained from Coriell Cell Repositories (Coriell Institute for Medical Research, Camden, NJ, USA). The HGPS cell lines AG10750 (M, 9 years), AG11498 (M, 14 years) and AG11513 (F, 8 years) have been shown to be heterozygous for the codon 608 GGC>GGT mutation (Erikkson et al., 2003). The age indicated in parentheses refers to the age of the donor at the time of biopsy. For controls we used primary cell lines derived from age-matched, apparently normal donors: GM00038C (F, 9 years), GM00316B (M, 12 years) and GM08398C (M, 8 years). Total RNA was isolated using the TRIzol reagent (Life Technologies) and further purified on RNeasy mini columns (Qiagen).Five micrograms of total RNA was used in each labeling reaction. Hybridization was done using Affymetrix HG-U133B arrays.
Data processing
Affymetrix HG-U133B arrays were used. Intensity values were quantitated by MAS 5.0 software (Affymetrix). All the chips were scaled to the same target intensity of 500.
