|
| Status |
Public on Mar 01, 2006 |
| Title |
MDER +Myog 12h replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblast transduced with MDER + Myog
|
| Organism |
Mus musculus |
| Characteristics |
cultured primary fibroblasts isolated from Myod-/- and Myf5-/- mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 35mm plates using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
6 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
MDER+Myog cells induced for 12h in DMEM+1%HS+Insulin+Transferin+beta-estradiol
|
| Data processing |
Bioconductor RMA package
|
| |
|
| Submission date |
Dec 16, 2005 |
| Last update date |
Dec 19, 2005 |
| Contact name |
Yi Cao |
| E-mail(s) |
[email protected]
|
| Phone |
206-667-5278
|
| Organization name |
Fred Hutchinson CRC
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview Ave. N
|
| City |
Seatttle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE3858 |
Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters |
|
